基于ATAC-seq和RNA-seq研究CWIN调控采后番茄果实耐冷性作用机制

批准号:
31801915
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
茹磊
依托单位:
学科分类:
C1509.园艺作物采后生物学
结题年份:
2021
批准年份:
2018
项目状态:
已结题
项目参与者:
李永新、曹雪慧、刘丽萍、张宏玲、陈月皎
国基评审专家1V1指导 中标率高出同行96.8%
结合最新热点,提供专业选题建议
深度指导申报书撰写,确保创新可行
指导项目中标800+,快速提高中标率
微信扫码咨询
中文摘要
低温贮藏是番茄果实保鲜最有效的方法,但番茄果实属于冷敏型果蔬,当温度低于12°C时,会发生冷害,可造成严重损失。蔗糖代谢不但可为植物抗逆反应提供能量,还可通过蔗糖代谢信号途径参与植物抗逆反应。研究表明,水解蔗糖的细胞壁蔗糖转化酶(CWIN)调控植物低温耐受性,但其作用机制尚不明确。本项目拟利用已获得的CWIN活性上调的转基因番茄为试材,首先解析CWIN调控番茄果实耐冷性的理化机制;然后利用转录组测序分析低温胁迫下CWIN调控的差异基因表达谱和生物学通路;再通过整合转录组和转座酶可接近性染色质测序分析结果,筛选出CWIN调控的番茄果实耐冷性形成的关键转录因子,并通过转基因技术对其功能进行验证。本研究将阐明CWIN调控番茄果实耐冷性作用机制,为采后果实低温冷害防治提供新思路。
英文摘要
Storage at low-temperature is the most effective practice to preserve tomato fruit quality. Tomato fruit is chilling-sensitive, which suffers chilling injury when stored at temperature lower than 12°C, resulting in serious loss. Sucrose metabolism not only provides energy for plant defense responses, but also acts as signals to regulate plant stress tolerance. Recent studies have shown that cell wall invertase (CWIN) plays a regulatory role in plant chilling tolerance, but the molecular pathway remains unclear. In this study, a transgenic tomato line with elevated CWIN activity and its wild type will be used to study the mechanism of chilling tolerance induced by elevated CWIN activity in tomato fruits. Firstly, biochemical basis of CWIN regulated chilling tolerance will be studied. Then, differentially expressed genes and molecular pathway will be unraveled by using RNA-seq. Finally, key transcription factors associated with CWIN induced chilling tolerance in tomato fruit will be screened by combing RNA-seq and ATAC-seq results. The role of key transcription factors will be functionally characterized. This project will unravel the molecular mechanism of CWIN regulated chilling tolerance in tomato fruit and provide new insight for prevention of chilling injury in tomato fruit during low-temperature storage.
本项目拟研究蔗糖转化酶在番茄冷害中的作用,但因转基因材料种子发芽率低,蔗糖转化酶活性变化减少,原计划研究内容未能全部完成。在本课题资助下,蔗糖转化酶相关糖转运蛋白被确定,研究结果表明糖转运蛋白SlHT2和SlSWEET12c响应蔗糖转化酶活性的改变。另外,蔗糖转化酶活性在植物发育和逆境胁迫中都发挥重要作用,研究调控其活性的机理将对植物正常发育及抵抗逆境胁迫起到重要的作用。研究结果证实SlINVINH2和SldeCWIN1为蔗糖转化酶的调控因子,我们重新创制了蔗糖转化酶活性调控因子的转基因材料,获得SlINVINH2和SldeCWIN1转基因植株。本项目所获研究结果为后续再次开展本项目的内容打好基础,并可拓展蔗糖转化酶活性调控的机理。
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
Integrating Sugar Metabolism With Transport: Elevation of Endogenous Cell Wall Invertase Activity Up-Regulates SlHT2 and SlSWEET12c Expression for Early Fruit Development in Tomato.
将糖代谢与运输结合起来:内源细胞壁转化酶活性的升高上调SlHT2和SlSWEET12c表达,促进番茄早期果实发育
DOI:10.3389/fgene.2020.592596
发表时间:2020
期刊:Frontiers in genetics
影响因子:3.7
作者:Ru L;He Y;Zhu Z;Patrick JW;Ruan YL
通讯作者:Ruan YL
DOI:https://doi.org/10.3389/fgene.2020.592596
发表时间:2020
期刊:Frontiers in Genetics
影响因子:3.7
作者:Lei Ru;Yong He;Zhujun Zhu;John Patrick;Yongling Ruan
通讯作者:Yongling Ruan
Chitosan oligosaccharides induced chilling resistance in cucumber fruit and associated stimulation of antioxidant and HSP gene expression
壳寡糖诱导黄瓜果实抗冷性以及相关的抗氧化剂和 HSP 基因表达刺激
DOI:10.1016/j.scienta.2020.109187
发表时间:2020-04-05
期刊:SCIENTIA HORTICULTURAE
影响因子:4.3
作者:Ru, Lei;Jiang, Lingfeng;Li, Yongxin
通讯作者:Li, Yongxin
DOI:--
发表时间:2020
期刊:渤海大学学报(自然科学版)
影响因子:--
作者:茹磊;王丽岩;王超;李永新
通讯作者:李永新
国内基金
海外基金
