人类和动物约60%的疾病是由病毒感染引起的。西医在抗病毒治疗上有一定局限性，而中医药抗病毒具有多重优势。研究特效抗病毒中药的分子作用机制，阐明其中的信号通路和宿主抗病毒因子，有助于揭示中药抗病毒的作用靶点，使中药靶向抗病毒治疗成为可能。APOBEC3G和BST-2是近期发现的受I型干扰素调控的抗病毒蛋白，抑制包括HIV-1在内的多种病毒感染及释放。本项目将通过分子生物学和细胞学手段，揭示人参皂苷调控TLR/RLR信号通路，活化下游IRF3/7信号分子并激活I型干扰素，诱导宿主抗病毒因子APOBEC3G和BST-2蛋白表达并发挥抗病毒功能的分子机制。同时，研究人参皂苷对APOBEC3G/BST-2蛋白修饰，细胞定位，病毒拮抗作用等方面的影响。本研究将有助于更好的了解人参有效成分，为开发新型人参制品和抗病毒中药提供理论依据，增强临床的可应用性。

About 60% of human and animal diseases are caused by viral infection. Western medicine has limitations in treating viral diseases, while traditional Chinese medicine (TCM) has multiple advantages in antiviral treatments. Study the molecular mechanism of antiviral TCM, clarify the related signaling pathways and host antiviral factors, is helpful to reveal the targets of antiviral TCM, increase the possibilities of targeted-TCM antiviral therapy. APOBEC3G and BST-2 are recently discovered antiviral proteins which regulated by the type I interferon, inhibiting the infection and release of various viruses including HIV-1. This project will investigagte the antiviral activity of the ginsenoside with the molecular biological and cytological methods, aimed to reveal the molecular mechanism that ginsenoside regulates TLR/RLR signaling pathway, activates the downstream IRF3/7 signaling molecule, stimulates the type I IFN and induces the expression of host antiviral factors APOBEC3G and BST-2. Meanwhile, this project will also study the effect of ginsenoside on modification, cellular localization and viral antagonism of APOBEC3G/BST-2. The study will contribute to a better understanding of active ingredients of ginseng, provide a theoretical basis for the development of new antiviral TCM drugs and ginseng products, and enhance the clinical applicability.