竞争性内源RNA（ceRNAs）通过调节microRNAs的生物活性在肿瘤的发生发展中发挥重要作用，ceRNAs在肿瘤转移中的作用机制研究是目前国内外研究热点之一。本课题组先前研究证实了miR-125b通过靶向STARD13促进上皮间质转化的发生从而提高乳腺癌转移能力。本项目进一步提出miR-125b有可能通过ceRNAs网络调控机制对乳腺癌转移起重要作用。为此，本项目拟开展以下研究：1）采用生物信息学方法、免疫共沉淀结合高通量测序等技术并结合临床样本分析确证miR-125b相关ceRNAs调控网络的存在；2）应用活细胞工作站、近红外活体成像等技术在体内外探讨miR-125b 相关ceRNAs网络对乳腺癌转移的调控作用；3）通过荧光素酶报告法、重组质粒构建等研究方法深入阐述microRNAs与ceRNAs网络对乳腺癌转移调控作用的新机制；从而为恶性肿瘤的临床诊断与治疗提供新的靶点。

Competing Endogenous RNAs (ceRNAs) play a critical role in tumorigenesis by regulating the bioactivities of microRNAs. The underlying mechanism of ceRNAs' regulative effects on cancer metastasis is one of the most heated topics. We previously proved that miR-125b promotes epithelial-mesenchymal transition (EMT) by targeting STARD13. Therefore miR-125b increases the metastatic ability of breast cancer cells. In this study, we furtherly assume that miR-125b affects breast cancer metastasis through ceRNA crosstalk. To prove this hypothesis, our team carries out the following research: 1) The existence of miR-125b-related ceRNA crosstalk is validated by utilizing bioinformatics and co-immunoprecipitation high-throughput sequencing. 2) Regulative effect of miR-125b-related ceRNAs crosstalk is investigated with the help of live cell station and near-infrared in vivo imaging. 3) By utilizing luciferase assay and recombinant plasmid, the new mechanisms of the interaction between microRNAs and ceRNA crosstalk, as well as its regulative effect on the metastasis of breast cancer, is in-depthly demonstrated. In this way, we make efforts to discover a new potential target for clinical diagnosis and treatment of cancer.