离子掺杂羟基磷灰石的微结构及其与BMP-7多肽相互作用机理的高场固体NMR研究

羟基磷灰石是构成人体骨骼和牙齿的主要无机成分，具有良好的生物活性和生物相容性，因此被广泛用作生物医学材料。金属离子掺杂羟基磷灰石的生物活性比纯的羟基磷灰石有显著的提高。高分辨固体核磁共振技术是研究天然骨骼和具有生物活性磷酸钙生物陶瓷的一种十分有效的方法。本项目拟采用高分辨固体核磁共振、原子力显微镜、X射线粉末衍射、扫描和透射电子显微镜、红外和拉曼光谱和理论计算等方法，研究离子掺杂羟基磷灰石的微结构。结合体外生物活性实验，揭示掺杂离子—微结构—生物活性之间的关系。更重要的是，本项目将以骨形态发生蛋白多肽与离子掺杂羟基磷灰石的相互作用为研究重点，通过高场固体NMR技术结合理论计算研究BMP-7活性多肽与羟基磷灰石，以及掺杂离子与多肽之间的相互作用，为骨形态发生蛋白的骨诱导机制提供理论依据，同时也为高活性离子掺杂羟基磷灰石的设计和开发提供指导。

Hydroxyapatite is the main mineral in teeth and bones within the human body. The excellent bioactivity and biocompatibility of hydroxyapatite make it a widely used biomedical material. Compared with pure hydroxyapatite, metal-ion-incorporation of hydroxyapatite results in materials with highly improved bioactive behavior. High-resolution solid-state NMR has been proved to be a powerful technique for studying the natural bone and biological relevant calcium phosphate ceramics. In this project, the microstructure of metal-ion-doped hydroxyapatite will be studied using a series of techniques including high-resolution solid-state NMR, atomic force microscopy, X-ray powder diffraction, scanning and transmission electron microscopy, FT-IR and Raman spectroscopy, and density function theory (DFT) calculation. Further in vitro bioactivity tests will be applied to reveal the microstructure-bioactivity relationship. Moreover, the study of the interaction between bone morphogenetic protein peptide fragment and metal ions-doped hydroxyapatite will provide a unique perspective in this project. Using solid-state NMR techniques in combination with DFT quantum chemical calculations, the interactions among BMP-7 active peptide, metal ions and hydroxyapatite will be studied. These results will not only provide theoretical foundation for understanding the osteoinduction mechanism induced by bone morphogenetic protein, but also offer guidance for designing new metal-ion-doped hydroxyapatite with higher bioactivity.