现代月季（Rosa hybrida）是蔷薇属内种间反复杂交和长期选育而形成的高度杂合的庞大品种群，优良品种主要是靠无性繁殖，而离体器官的形态建成就成了为无性繁殖的关键。课题组多年研究发现了一个现代月季（Rdn-1）株系，其能高频、快速地在离体茎段的叶腋处同时产生芽和根，形成完整植株，因此为木本植物器官发生的分子机理研究提供了难得的实验材料和必要条件。本研究在解剖学初步的研究基础上，通过形态、细胞学、生理生化研究结合现代分子研究技术，利用基因组测序、生物信息学分析及蛋白质学等技术，研究器官形态建成过程的关键基因及其调控细胞及器官发生的动态变化规律，挖掘和验证关键调控基因的功能，分析关键转录因子下游调控网络和互作蛋白，探讨细胞、激素、基因和蛋白协同调控关系，建立现代月季调控不定根发育的分子网络模型，初步揭示其叶腋生根机理及分子调控机制，为木本蔷薇类植物快速繁育提供理论和实践支撑。

Rosa hybrida is from repeatedly interspecific hybridization which formed a huge number of hybrids by selection and breeding during a long term. Morphogenesis of isolated organs is the key of asexual reproduction, which is the main way to generate clone lines for roses. Due to the previous research of Rosa hybrida, we found a hybrid line could form new leaf and root at axil bud under certain condition that called Rdn-1, which might to be a valuable material to study on the molecular mechanism of organogenesis in woody plants. This research tends to mine the key genes and their function on morphogenesis and dynamic changes of cells and organs via morphology, cytology, physiology, biochemistry and molecular biology based on the anatomy. Biological technical methods including trascriptome analysis, informatics, plant molecular biology and proteomics technology will be used to detect the development processes in Rosa hybrida. The study will also focus on the downstream regulatory networks and interaction proteins of key transcript factors (TFs). Above all try to clarify synergistic regulation among cells, hormones, genes, proteins in adventitious roots (ARs) development. Finally, it will claim the reason of ARs development at leaf axil and uncover mechanisms of young woody plants forming, which will provide a basic for organ forming and woody plant rapid reproduction research of woody plants.