硫醚类化合物介导大蒜根系“attract-kill”控治疫病的细胞程序化死亡作用机制研究

批准号:
31972328
项目类别:
面上项目
资助金额:
57.0 万元
负责人:
刘屹湘
依托单位:
学科分类:
生物防治
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
刘屹湘
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中文摘要
非寄主植物根系吸引土传病菌孢子使其迅速休止并裂解,丧失传播侵染能力(“attract-kill”)控病模式,是多样性种植控制土传病害的重要机制。申请人前期研究发现,硫醚类化合物是大蒜根系抑制辣椒疫霉菌侵扰的关键抑菌物质,其抑菌过程还伴随细胞凋亡和自噬程序化死亡信号通路激活。本研究据此探究硫醚类化合物介导大蒜根系“attract-kill”控疫病模式中细胞程序化死亡作用机制。拟借助荧光染色、化学药物筛选、CRISPR/Cas9技术等方法,探明细胞程序化死亡与硫醚类化合物抑菌机制的关系和贡献;筛选和验证硫醚类化合物诱导细胞程序化死亡的关键基因;验证关键调控基因在其他非寄主植物根系“attract-kill”抑菌模式中的普适性。预期研究结果将为生物多样性化学生态学控病机理提供新的理论依据;凋亡和自噬关键基因有望作为抑制疫霉菌潜在作用靶点,对病害防控新药剂的研发具有重要生产指导意义。
英文摘要
The "attract-kill" pattern by non-host plant root system, which could attract zoospores of soil-borne pathogen and induced rupture, is an important mechanism in the suppression of soil-borne diseases in diversity planting. Our recent research indicated the sulphur compounds were the important antifungal substances for garlic roots to interfere the infection process of phytophthora capsicum. The dynamic process of inhibition was also accompanied by the signaling pathway activation of programmed cell death (apoptosis and autophagy), which may be an important mechanism in inhibition for soil-borne pathogen of sulphur compounds. This project using the method of fluorescent staining, inhibitor and Crispr/Cas9 to research the antifungal mechanism of programmed cell death in the "attract-kill" pattern mediated by sulphur compounds involved in suppression of phytophthora disease by garlic roots as follows: ① study the relationship between programmed cell death and the antifungal mechanism of sulphur compounds by fluorescent staining; ② analyze the importance of programmed cell death in the antifungal mechanism of sulphur compounds by screening the inhibitors of programmed cell death of Phytophthora capsici; ③ verify the functions of key genes of programmed cell death in the antifungal mechanism of sulphur compounds by CRISPR/Cas9 technology; ④ analyze the universality of key genes of programmed cell death in other "attract-kill" pattern. The results are expected to provide a new theoretical basis for chemical ecology mechanism in disease control by biodiversity. And the key genes of apoptosis and autophagy can also be potential targets for inhibition of phytophthora, which is important to research new pesticide to inhibit soil-borne disease.
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专利列表
DOI:--
发表时间:2021
期刊:Frontiers of Agricultural Science and Engineering
影响因子:3.7
作者:Yuxin Yang;He Zhang;Yuting Fang;Ying Li;Xinyue Mei;Huichuan Huang;Fei Du;Shusheng Zhu;Ming Yang;Yixiang Liu
通讯作者:Yixiang Liu
DOI:--
发表时间:2022
期刊:云南农业大学学报(自然科学)
影响因子:--
作者:李颖;何依依;孙俊薇;张治萍;梅馨月;王海宁;刘屹湘;李作森
通讯作者:李作森
DOI:10.3389/fpls.2022.890534
发表时间:2022
期刊:FRONTIERS IN PLANT SCIENCE
影响因子:5.6
作者:Yang, Yuxin;Li, Ying;Mei, Xinyue;Yang, Min;Huang, Huichuan;Du, Fei;Wu, Jiaqing;He, Yiyi;Sun, Junwei;Wang, Haining;He, Xiahong;Zhu, Shusheng;Li, Yingbin;Liu, Yixiang
通讯作者:Liu, Yixiang
DOI:10.1111/ppa.13550
发表时间:2022
期刊:Plant Pathology
影响因子:--
作者:Yingbin Li;Yixiang Liu;Zhiping Zhang;Kaiyuan Shen;Jianjun Hao;Laixin Luo;Jianqiang Li
通讯作者:Jianqiang Li
DOI:10.3389/fpls.2020.00886
发表时间:2020-07
期刊:Frontiers in Plant Science
影响因子:5.6
作者:He Zhang;Yuxin Yang;Xinyue Mei;Ying Li;Jiaqing Wu;Yiwen Li;Huiling Wang;Huichuan Huang
通讯作者:He Zhang;Yuxin Yang;Xinyue Mei;Ying Li;Jiaqing Wu;Yiwen Li;Huiling Wang;Huichuan Huang
大蒜根泌硫化物塑造根际微生物群落协同控制辣椒疫病作用机制
- 批准号:--
- 项目类别:地区科学基金项目
- 资助金额:33万元
- 批准年份:2022
- 负责人:刘屹湘
- 依托单位:
间作体系中非寄主植物根系“attract-kill”模式控制疫病的化学生态学机制
- 批准号:31601682
- 项目类别:青年科学基金项目
- 资助金额:20.0万元
- 批准年份:2016
- 负责人:刘屹湘
- 依托单位:
国内基金
海外基金
