非寄主植物根系吸引土传病菌孢子使其迅速休止并裂解，丧失传播侵染能力（“attract-kill”）控病模式，是多样性种植控制土传病害的重要机制。申请人前期研究发现，硫醚类化合物是大蒜根系抑制辣椒疫霉菌侵扰的关键抑菌物质，其抑菌过程还伴随细胞凋亡和自噬程序化死亡信号通路激活。本研究据此探究硫醚类化合物介导大蒜根系“attract-kill”控疫病模式中细胞程序化死亡作用机制。拟借助荧光染色、化学药物筛选、CRISPR/Cas9技术等方法，探明细胞程序化死亡与硫醚类化合物抑菌机制的关系和贡献；筛选和验证硫醚类化合物诱导细胞程序化死亡的关键基因；验证关键调控基因在其他非寄主植物根系“attract-kill”抑菌模式中的普适性。预期研究结果将为生物多样性化学生态学控病机理提供新的理论依据；凋亡和自噬关键基因有望作为抑制疫霉菌潜在作用靶点，对病害防控新药剂的研发具有重要生产指导意义。

The "attract-kill" pattern by non-host plant root system, which could attract zoospores of soil-borne pathogen and induced rupture, is an important mechanism in the suppression of soil-borne diseases in diversity planting. Our recent research indicated the sulphur compounds were the important antifungal substances for garlic roots to interfere the infection process of phytophthora capsicum. The dynamic process of inhibition was also accompanied by the signaling pathway activation of programmed cell death (apoptosis and autophagy), which may be an important mechanism in inhibition for soil-borne pathogen of sulphur compounds. This project using the method of fluorescent staining, inhibitor and Crispr/Cas9 to research the antifungal mechanism of programmed cell death in the "attract-kill" pattern mediated by sulphur compounds involved in suppression of phytophthora disease by garlic roots as follows: ① study the relationship between programmed cell death and the antifungal mechanism of sulphur compounds by fluorescent staining; ② analyze the importance of programmed cell death in the antifungal mechanism of sulphur compounds by screening the inhibitors of programmed cell death of Phytophthora capsici; ③ verify the functions of key genes of programmed cell death in the antifungal mechanism of sulphur compounds by CRISPR/Cas9 technology; ④ analyze the universality of key genes of programmed cell death in other "attract-kill" pattern. The results are expected to provide a new theoretical basis for chemical ecology mechanism in disease control by biodiversity. And the key genes of apoptosis and autophagy can also be potential targets for inhibition of phytophthora, which is important to research new pesticide to inhibit soil-borne disease.