外泌体介导细胞通讯、免疫反应和物质运输等重要生命过程，其中蛋白质是外泌体行使功能的关键物质基础。针对外泌体及其蛋白质的分子特征开展靶向分析，对于生命过程认知和疾病检测具有重要意义。本项目拟以具有生理病理特征意义的外泌体及相关蛋白质为靶标，设计、合成并筛选出高亲和力、高选择性的识别多肽；从协同效应的角度出发，构建新型印迹纳米材料；以多肽或印迹材料为识别元件，发展具有识别信号响应的探针；建立外泌体及相关蛋白质的高选择性分离新方法和传感分析新体系；探索获取肿瘤等病变过程中外泌体和蛋白质的变化信息，为外泌体和相关蛋白质的结构和功能解析、疾病的检测提供新工具和新方法。

Exosomes mediate important biological processes including cell communication, immune reactions and transportation, whereas exosomal proteins lay the foundation for these functions. Targeted analysis of exosomes and related proteins is crucial for understanding the nature of life and disease detection. In this proposal, exosomes and proteins associated with particular physiological and pathological process were chosen as targets. Peptides with high affinity and specificity towards exosomes and related proteins will be designed, synthesized and screened. With the guidance of synergistic effect, novel imprinted nanomaterial will be developed. By using the affinity peptides or imprinted nanomaterial as recognition elements, the probes with target recognition-induced signal output will be fabricated. Analytical methods for highly selective separation and sensing of exosomes and related proteins will be established. The potential applications of imprinting-based materials and probes for analyzing caner-specific exosomes and proteins will be investigated. This study can provide new approaches for understanding the structure-function relationship of exosomes and related proteins. The affinity peptides and nanomaterial are also promising for disease detection.