tRNA碎片对IUGR仔猪肌肉代谢综合征的表观调控

仔猪宫内发育迟缓（Intra-Uterine Growth Restriction, IUGR）引起的高死亡率和发病率一直是制约我国猪业发展的一大“瓶颈”，然而IUGR形成的分子机理目前尚不清楚。最新研究表明tRNA衍生片段（tRFs）作为一种新型的表观遗传分子，具有类似miRNAs调控靶基因表达的功能。申请人在已有对IUGR猪的表观调控和tRFs作用机制的研究基础上，通过构建正常和IUGR仔猪肌肉的tRFs转录组图谱；筛选和鉴定与IUGR猪肌肉代谢综合征相关的tRFs；并在细胞和活体水平验证tRFs参与肌肉的葡萄糖耐受、线粒体生成、成肌分化的生物学功能，从而在多个层面论证tRFs是IUGR形成的新型表观调控分子。本研究成果将进一步完善和补充猪IUGR形成的表观遗传调控机制，有利于养猪业生产效益的提高，也为人类IUGR的研究提供了新的研究思路。

The high mortality and morbidity caused by Intra-Uterine Growth Restriction (IUGR) have always been a major restraining factor in the development of China's pig industry. However, the molecular mechanism of IUGR biogenesis is not well characterized yet. Recent studies have shown that tRNA-derived fragments (tRFs) can act as a novel epigenetic molecule, which has similar functions as miRNAs on regulating target gene expression. Based on previous studies about epigenetic regulation of IUGR pigs and molecular mechanism of tRFs, the applicant intend to construct tRFs transcriptome maps of both normal and IUGR piglets’ muscle tissue, and identify tRFs associated with IUGR pig’s muscle metabolism syndrome. Furthermore, in vivo and in vitro experiments would be used to verify that tRFs participate in the biological processes of glucose tolerance of IUGR muscle, mitochondriopoiesis, and myoblast differentiation. All these would help to prove that tRFs are novel epigenetic regulatory molecules in IUGR biogenesis. The research will further improve and supplement the epigenetic regulation mechanism of pig IUGR biogenesis, which is conducive to the improvement of pig production efficiency, and also provides new research ideas for human IUGR research.