Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model.
Characteristics of tRNA-Derived Small RNAs and microRNAs Associated with Immunocompromise in an Intrauterine Growth-Restricted Pig Model.
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tRNA 衍生的小 RNA 和 microRNA 与宫内生长受限猪模型中免疫功能低下相关的特征
DOI：
10.3390/ani12162102
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发表时间：
2022-08-17
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影响因子：
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宫内生长受限（IUGR）是指胚胎或胎儿在哺乳动物子宫内生长发育缓慢。IUGR新生儿通常表现为生长缓慢，身体和器官的发育伴随着生命早期感染风险的增加。IUGR仍然是一个重大的全球公共卫生问题，特别是在发展中国家。在这项工作中，我们研究了转移RNA衍生的小RNA和microRNA在脾脏中的表达谱使用猪作为IUGR模型。这些结果揭示了一个重要的潜在调节网络参与IUGR引起的免疫功能低下。本研究为IUGR的分子调控机制提供了新的视角，为IUGR的防治提供了参考。宫内生长受限（IUGR）是哺乳动物新生儿发病和死亡的重要原因。转移RNA衍生的小RNA（transferRNA-derivedsmallRNA，tsRNA）是近年来出现的一种非编码RNA。tsRNA和microRNA（miRNAs）具有相似的机制，参与多种生物学过程。本研究以猪为IUGR模型，采用RNA测序技术对脾脏中的tsRNA和miRNA表达谱进行了分析。共鉴定出361个miRNAs和620个tsRNAs，其中差异表达的miRNAs（DEM）22个，差异表达的tsRNAs（DET）25个。tRF-5c是主要的tsRNA类型，占90%以上，其中以tRNA-Gly-GCC表达量最高。功能富集分析发现，这些DET和DEM与免疫系统过程有关。蛋白质-蛋白质相互作用（PPI）网络分析显示ssc-miR-370、ssc-miR-206、tiRNA-Ser-TGA-001和tRF-Val-AAC-034可能是主要的调节因子。TNF、TLR 4、CD 44、MAPK 1和STAT 1是预测的中枢靶基因。这些DET和DEM可能通过调节T细胞受体信号通路和Toll样受体信号通路介导IUGR引起的免疫功能低下。本文讨论的结果揭示了tsRNAs和miRNAs在IUGR猪脾脏中的潜在作用。
Intrauterine growth restriction (IUGR) refers to the slow growth and development of an embryo or fetus in the uterus of mammals. IUGR newborns commonly present with slow growth and the development of the body and organs accompany increased risks of infection during the early life period. IUGR remains a significant global public health issue, particularly in developing countries. In this work, we investigated the transfer RNA-derived small RNA and microRNA expression profiles in the spleen using pigs as an IUGR model. These results uncover an important potential regulator network involved in immunocompromise caused by IUGR. The present studies provide a novel perspective on the molecular regulatory mechanism of IUGR and a reference for prevention and treatment. Intrauterine growth restriction (IUGR) is an important cause of newborn morbidity and mortality in mammals. Transfer RNA-derived small RNA (tsRNA) has become an emerging non-coding RNA in recent years. tsRNA and microRNAs (miRNAs) share similar mechanisms, which are involved in various biological processes. In this study, the pig was used as a model of IUGR, and the tsRNA and miRNA expression profile in the spleen was characterized by RNA sequencing. A total of 361 miRNAs and 620 tsRNAs were identified, of which 22 were differentially expressed miRNA (DEM) and 25 differentially expressed tsRNA (DET). tRF-5c were the primary tsRNA type making up more than 90%, and the most abundantly expressed tsRNAs are from tRNA-Gly-GCC. Functional enrichment analysis found that those DETs and DEMs have been implicated in the immune system process. Protein–protein interaction (PPI) network analysis revealed ssc-miR-370, ssc-miR-206, tiRNA-Ser-TGA-001 and tRF-Val-AAC-034 could be major regulators. TNF, TLR4, CD44, MAPK1 and STAT1 were predicted hub target genes. Those DETs and DEMs may regulate the T-cell receptor signaling pathway and Toll-like receptor signaling pathway to mediate the immunocompromise caused by IUGR. The results discussed in this article uncover the potential role of tsRNAs and miRNAs in IUGR porcine spleen.
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