在恒温下既可实现超灵敏核酸检测的等温核酸扩增是基因诊断学中最有望实现便携式定点护理诊断（POC）的技术手段。但其从实验室到医疗诊断部门，甚至到POC的真正转化却一直受限于 “缺乏特异性信号传导平台”这一瓶颈。本项目拟利用基于核酸链交换反应的核酸分子循环线路作为等温核酸扩增和信号输出之间的特异性枢纽。通过环介导等温扩增（LAMP）与核酸分子循环线路的高效偶联建立起针对致病基因的超灵敏、超特异“普适型”分子识别和信号传导平台，最大程度的消除“假像”信号；并采用实时荧光和血糖仪读数两种信号表征方式证明该偶联方法直接用于实验室和医疗单位的可能性，以及用于实现POC诊断的巨大潜力。具体模型分析对象将首先采用目前最受关注的埃博拉病毒基因，随即推广到其他重大疾病相关的基因检测上。

A series of isothermal nucleic acid amplification reactions are considered as the most promising techniques that can lead to point-of-care (POC) gene diagnosis. However, their real applications in clinical departments or POC fields have been badly delayed by lacking of sequence-specific downstream signaling methods. In this project, we propose to use strand exchange based nucleic acid amplification circuit as an ultra-specific and smart bridge between isothermal amplifications and final readouts. By using nucleic acid amplification circuit to probe loop mediated isothermal nucleic acid amplifications, the “false signal” frequently raised by traditional detection methods will be eliminated, and an ultra-sensitive and ultra-specific universal diagnosis platform will be established for widely concerned diseases. Both real-time florescence and commercial glucometer will be adapted as final readouts, which will finally demonstrate the promising potential for real applications of such LAMP-circuit system in lab, clinic, and even POC. The model target will start from gene of Ebola virus and be generalized to other widely concerned diseases.