villin调控植物生长发育的机理研究

微丝骨架动态组装参与调控许多基本的细胞生理学过程。微丝结合蛋白是微丝骨架动态组装的最直接调控者，阐明其作用机制及其本身活性的调节方式有助于理解微丝骨架动态组装调控机理及微丝骨架行使功能的分子基础。Villin是一类微丝细胞骨架动态组装调控因子，参与调控了花粉管和根毛生长等重要的细胞生理学过程。植物编码多个villin基因并在不同部位表达，它们的功能如何分化以适应不同的细胞生理学功能了解甚少。此外，由于villin具有多种微丝骨架动态组装调控活性，它如何在这些生理学过程中精确发挥功能也了解甚少。还有，villin如何在微丝动态组装和信号转导途径偶联的过程中扮演角色也有待于阐明。本项目将对上述问题展开研究，预期将增进理解villin行使功能的作用机制。相关研究对于理解微丝骨架动态组装调控的分子机制以及微丝骨架调控植物生长发育的分子机理具有重要的科学理论意义。

Dynamic assembly and disassembly of the actin cytoskeleton has been implicated in numerous fundamental physiological cellular processes, which is strictly regulated in cells. Actin-binding proteins (ABPs) are direct regulators of the dynamic assembly and disassembly of actin, elucidation of the mechanism of action and mode of regulation of those ABPs is going to enrich our understanding of the function and mechanism of action of the actin cytoskeleton. Villin is one type of actin regulatory proteins that is involved in numerous physiological cellular processes, including pollen tube growth and root hair growth. Multiple villin isovariants are encoded in plant genome that exhibit distinct expression patterns, how those villin isovariants evolve to fulfill different physiological cellular functions remains largely unclear. In addition, given that villin has multiple actin regulatory functions, how it exactly functions during those physiological processes remains incompletely understood. Furthermore, how villin, as a signal-responsive ABP, integrates actin dynamics into various signal transduction pathways remains largely unknown. This project aims to address these unanswered questions, which promises to reveal the molecular mechanism underlying the action of villin in the regulation of plant growth and development, as well as the mechanism of action of the plant actin cytoskeleton in this aspect in general.