剑麻IAA6转录因子调控纤维发育功能研究
结题报告
批准号:
32001598
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
黄兴
学科分类:
作物基因组及遗传学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
黄兴
国基评审专家1V1指导 中标率高出同行96.8%
结合最新热点,提供专业选题建议
深度指导申报书撰写,确保创新可行
指导项目中标800+,快速提高中标率
客服二维码
微信扫码咨询
中文摘要
剑麻是我国热带地区重要叶纤维作物,其叶片生物量大但含麻率较低,严重制约剑麻纤维生产。项目组前期通过转录组比较揭示出野生剑麻向栽培剑麻进化机制,筛选出仅在主栽品种H11648中受正向选择(Ka/Ks>1)的IAA6转录因子。模式植物中Aux/IAA转录因子家族相关研究主要集中在生长素信号应答,其调控纤维发育功能研究相对较少。本研究在开展了剑麻叶片不同发育时期组织学观察,基本明确纤维发育形态变化特征与IAA6表达模式存在相关性的基础上,克隆IAA6全序列并构建过表达载体,以项目组建立的遗传转化体系转化剑麻,筛选转基因阳性植株并进行纤维相关表型鉴定。同时通过酵母双杂交筛选IAA6互作蛋白,并对未转化和转基因植株转录组测序以解析纤维发育过程中的基因表达差异,据此构建基于IAA6转录因子的剑麻纤维发育基因调控网络。研究结果将为剑麻纤维发育分子机制研究提供理论依据,为纤维性状遗传改良提供基因资源。
英文摘要
Sisal is one of the most important leaf fiber crops in tropical area of China. Sisal leaf has large biomass with low fiber content, which has significantly restricted sisal fiber production. We previously revealed the selection pattern of wild and domesticated agave species by transcriptome comparison and obtained a transcription factor named IAA6, which is subjected to positive selection (Ka/Ks>1) in agave main cultivar H11648. In model plants, the research topic of Aux/IAA transcription factor family mainly focused on auxin signaling response. In contrast, only a few were related to fiber development. In the present study, we have conducted histological and IAA6 expression analysis of sisal leaves at different developmental stages, which has indicated the correlations between morphological characteristics and IAA6 expression during the process of fiber development. We will further clone the full sequence of IAA6 by RACE for the construction of overexpression vectors, which will be transformed to sisal with our successful genetic transformation system. Transgenic plants will be screened by PCR and Southern blot and characterized with fiber related traits. The proteins interacted with IAA6 will be examined by yeast two-hybrid assay. And a transcriptome comparison will be conducted between transgenic and non-transgenic plants for constructing IAA6 mediated gene network during fiber development of sisal. The results will provide theoretical basis for the molecular mechanism of sisal fiber development and gene information for the improvement of fiber traits.
专著列表
科研奖励列表
会议论文列表
专利列表
国内基金
海外基金