由核桃炭疽病菌引起的核桃炭疽病严重危害着我国核桃的健康生产，而其成灾机制尚不明确，严重制约着新型农药的开发。本研究从进化、时间、空间、表达量四个角度解析核桃炭疽病菌侵染核桃过程中糖苷水解酶蛋白家族GH协同作用机制，为进一步明确核桃炭疽病在田间成灾的“内因”机制提供重要的理论基础，为今后实现农药作用靶标找寻、设计等基础研究成果向应用开发提供重要的理论依据和支撑。进化：基于20种不同营养类型的真菌及不同种类微生物中GH信息，利用比较基因组学明确彼此之间GH的种类、数量、选择压力、遗传关系等异同；时间：利用RNA-seq测序技术分析核桃炭疽病菌生长发育及不同侵染阶段的GH表达模式；空间：通过对20个GH蛋白亚家族开展基因敲除、农杆菌介导的PVX双元载体在本氏烟的表达系统、原核表达系统分析其基因功能及亚细胞定位；表达量：通过对不同时间、空间表达模式的GH相关基因功能组合分析进一步明确GH协同作用。

Walnut anthracnose caused by Colletotrichum gloeosporioides seriously endangers the healthy production of walnut in China, but the catastrophe mechanism is still unclear, which seriously restricts the development of new pesticides. Therefore, the synergistic mechanism of glycoside hydrolase protein family (GH) during walnut anthracnose pathogen infecting stage were analyzed from four perspectives including evolution, time, space and expression, which can provide an important theoretical basis to further clarify the internal mechanism of walnut anthracnose disaster in the field, and provide an important theoretical basis for transforming basic theoretical research achievements such as finding and designing of pesticide targets into application and development. Evolution: Based on protein sequence of GH in 20 different trophic fungi and different types microorganisms, comparative genomics was used to clarify the similarities and differences of GH subfamily, number, selection pressure and genetic relationship among them. Time: GH expression patterns were analyzed by RNA-seq sequencing in the growth and development and different infection stages of walnut anthracnose pathogen; Space: The gene function and subcellular localization of 20 GH protein subfamilies were identified by gene knockout and Agrobacterium-mediated PVX binary vector expression system in Nicotiana benthamiana, prokaryotic expression system; respectively. Expression: Through the analysis of GH-related gene function combination in different time and space expression patterns, further clarify GH synergy function.