水翁花中新颖间苯三酚类成分的发现及其抗Ⅰ型单纯疱疹病毒作用机制和构效关系研究

批准号:
82003610
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
唐维
依托单位:
学科分类:
天然药物化学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
唐维
国基评审专家1V1指导 中标率高出同行96.8%
结合最新热点,提供专业选题建议
深度指导申报书撰写,确保创新可行
指导项目中标800+,快速提高中标率
微信扫码咨询
中文摘要
I型单纯疱疹病毒(HSV-1)是引起人类多种传染性疾病的常见病原体之一。以阿昔洛韦(ACV)为代表的病毒DNA聚合酶抑制剂是目前治疗HSV-1的首选方案,但该类药物易引起病毒耐药,已成为临床治疗面临的新难题,亟需研发新型的抗HSV-1药物。前期,本课题组从岭南中草药水翁中分离鉴定了一系列结构新颖的间苯三酚类成分,包括多个新骨架化合物。活性研究显示,该类化合物在体内外均具有显著的抗HSV-1活性,并对ACV耐药病毒株也表现出显著的抑制作用。初步的作用机制研究表明,该类化合物可显著降低病毒基因的表达,但与ACV不同,其抗HSV-1的作用机制可能与干扰病毒mRNA的出核转运有关,然而其具体的作用机制和作用靶标尚不清楚。本项目拟在前期工作基础上,进一步分离和富集水翁花中的间苯三酚类成分,并对其抗HSV-1的活性、作用机制和构效关系进行系统研究,为开发新型的抗HSV-1药物提供先导化合物和科学依据。
英文摘要
Herpes simplex virus type 1 (HSV-1) is one of the common human pathogens, which can lead to various infectious diseases. The viral DNA polymerase inhibitors, such as acyclovir (ACV), are considered as the first-choice drugs for the treatment of HSV-1 associated diseases. However, drug-resistance caused by these inhibitors has become a rising challenge in clinical. Hence, there is an urgent need for the development of new anti-HSV-1 drugs with novel antiviral mechanisms. Previously, we isolated and identified a series of new phloroglucinols with diversity structures from Cleistocalyx operculatus, including several compounds with novel carbon skeletons. The biological assay results showed that the isolated phloroglucinols potently inhibited the cytopathic effects and plaque formation in HSV-1-infected cells. Preliminary antiviral mechanism study demonstrated that phloroglucinols could suppress the expression of viral genes, and show a significant inhibitory effect on ACV-resistant virus, suggesting a distinctive antiviral mechanism of these phloroglucinols. The further investigation suggested that the proteins involved in viral mRNA export from the cell nucleus to cytoplasm might be the potential target of phloroglucinols against HSV-1. To provide leading compounds and scientific basis for the development of novel anti-HSV-1 agents, further study will be focused on the isolation of phloroglucinols with anti-HSV-1 activity from the buds of Cleistocalyx operculatus, as well as their mechanism of action and structure-activity relationship.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
DOI:10.1080/10286020.2023.2288290
发表时间:2024-01
期刊:Journal of Asian Natural Products Research
影响因子:1.7
作者:Jian-Guo Song;Jia-Xin Liu;Rui-Li Huang;Wei Tang;Xiao‐Jun Huang;Ying Wang;Wen-Cai Ye
通讯作者:Jian-Guo Song;Jia-Xin Liu;Rui-Li Huang;Wei Tang;Xiao‐Jun Huang;Ying Wang;Wen-Cai Ye
DOI:10.1002/anie.202312568
发表时间:2023
期刊:Angewandte Chemie International Edition
影响因子:--
作者:Wang Jie;Song Jian-Guo;Zhong Dong-Lin;Duan Zhi-Zhang;Peng Zi-Jian;Tang Wei;Song Qiao-Yun;Huang Xiao-Jun;Hu Li-Jun;Wang Ying;Ye Wen-Cai
通讯作者:Ye Wen-Cai
DOI:10.1016/j.fitote.2022.105229
发表时间:2022
期刊:Fitoterapia
影响因子:3.4
作者:Tan Ling-Xuan;Xia Tian-Qi;He Qi-Fang;Tang Wei;Huang Xiao-Jun;Song Qiao-Yun;Li Yao-Lan;Ye Wen-Cai;Wang Ying;Wu Zhen-Long
通讯作者:Wu Zhen-Long
DOI:10.1128/jvi.01205-21
发表时间:2021-09-27
期刊:Journal of virology
影响因子:5.4
作者:Tang W;Li Y;Song Q;Wang Z;Li M;Zhang Q;Wang Y;Ye W;Li Y
通讯作者:Li Y
DOI:doi.org/10.1021/acs.joc.2c00071 Lu-Ming Deng, Wei Tang, Shu-Qin Wang, Jian-Guo Song, Xiao-Jun Huan
发表时间:2022
期刊:Journal of Organic Chemistry
影响因子:--
作者:Deng Lu-Ming;Tang Wei;Wang Shu-Qin;Song Jian-Guo;Huang Xiao-Jun;Zhu Hao-Yue;Li Yao-Lan;Ye Wen-Cai;Hu Li-Jun;Wang Ying
通讯作者:Wang Ying
基于F/IGF1R/PKC ζ 通路研究夏枯草中
Mesonolic acid B抑制RSV感染性肺炎的
作用机制
- 批准号:--
- 项目类别:省市级项目
- 资助金额:10.0万元
- 批准年份:2025
- 负责人:唐维
- 依托单位:
国内基金
海外基金
