近年来，氯代多氟烷基醚磺酸钾（F-53B）和全氟壬烯氧基苯磺酸钠（OBS）作为全氟化合物（PFASs）替代品在水环境中广泛检出且浓度较高，但有关F-53B尤其OBS的环境行为、潜在动物和人体暴露风险数据依然缺乏。针对以上现状，本项目在前期研究基础上以我国主要河流中F-53B和OBS的检出浓度为参考暴露剂量，对斑马鱼进行世代暴露，探讨它们在鱼体的富集特征、组织分配和母体传递规律；从形态学发育终点、摄食量、耗氧率、alamar blue代谢率和运动行为等方面考察对鱼类摄食和能量代谢的影响；进一步通过对线粒体损伤相关指标（如线粒体膜电位、呼吸链复合物活性等）的检测并运用qRT-PCR、Western blot、代谢组学和分子计算模拟等技术手段，阐释F-53B和OBS干扰斑马鱼及其子代摄食和能量代谢的分子机制。研究成果将为准确评估新型PFASs替代品的水生生态风险和制定有效的管理对策提供科学依据。

In recent years, 6:2 chlorinated polyfluorinated ether sulfonate (F-53B) and sodium p-perfluorous nonenoxybenzenesulfonate (OBS), as substitutes for perfluoroalkyl substances (PFASs), have been widely detected in aquatic environment with high detection levels. However, the data on environmental behavior, potential animal and human exposure risks of F-53B and OBS are still lacking. In this study, the concentration of F-53B and OBS in the main rivers of China will be selected as the reference exposure does, and zebrafish are exposed to these novel PFASs alternatives for three generations. Their bioaccumulation characteristics, tissue distribution and maternal transmission rules in fish, and the effects on morphometric end points, food intake, oxygen consumption rate, alamar blue metabolic rate and swimming behavior are studied to investigate the impact of the novel PFASs alternatives on fish feeding and energy metabolism. Furthermore, the molecular mechanisms will be interpreted by detecting the changes in mitochondrial damage related indicators (mitochondrial membrane potential and respiratory chain complex activity) and using qRT-PCR, Western blot, metabolomics and molecular simulation methods. The results of this study will provide a scientific basis for accurately assessing the aquatic ecological risks of novel PFASs alternatives and developing effective management strategies.