THF1调控光系统II不同复合体之间动态平衡的机理研究

高等植物的光系统II(PSII)是由PSII核心复合体与外周天线（LHCII）组成的超大分子复合体。随着环境条件的变化，PSII-LHCII复合体会发生重组,其动态变化直接参与光系统之间的状态转换、非光化学淬灭以及反应中心D1蛋白的修复等重要生理过程。我们前期的研究结果表明在光合放氧生物中高度保守的PSII外周蛋白Thylakoid Formation 1 (THF1)在拟南芥中参与PSII-LCHII不同复合体之间的动态平衡。本项目将深入研究THF1调控PSII-LHCII动态变化的分子机理。我们将运用结构生物学、遗传学以及分子生物学等手段研究：1）THF1的晶体结构与生化功能；2）在逆境条件下THF1调控PSII-LHCII重组的功能保守性与THF1的作用机理；3）鉴定与THF1互作的新分子。本项目的研究成果将为今后运用生物技术提高作物抗逆性与光能利用效率提供新的思路。

In higher plants, photosystem II (PSII) is a large pigment–protein supramolecular complex composed of the PSII core complex and the plant-specific peripheral light-harvesting complexes (LHCII). PSII–LHCII complexes are highly dynamic in their quantity and macro-organization under various environmental conditions. THF1 (THYLAKOID FORMATION1) is a nuclear-encoded chloroplast protein highly conserved in oxygenic photosynthetic organisms, from cyanobacteria to vascular plants. In our previous study, we found that THF1 is involved in controlling PSII–LHCII dynamics during dark-induced senescence and light acclimation in Arabidopsis. This project will continue to investigate molecular mechanisms underlying THF1-regualted PSII-LHCII dynamic changes in response to environmental cues. We will mainly investigate the following aspects using structural, genetic and molecular biological approaches: 1) THF1 structure and biochemical function; 2) the molecular mechanism for THF1-regulated PSII-LHCII dynamic change; 3) identification of novel THF1-interacting proteins. Our results will provide new ideas to improve crop resistance to stresses and photosynthetic efficiency using biotechnology in the future.