通过调控羟基含量构建梯度渐变润湿性钛表面及其生物学效应研究

超亲水表面的口腔种植体在临床上的成功应用提示：赋予种植体适宜的表面润湿性是提高其骨整合性能的有效手段。但目前关于种植体润湿性梯度变化对其生物学性能的影响的研究鲜有报道，最佳的润湿性参数尚不明确。基于此，本项目首先解决钛种植体表面梯度润湿性的构建问题：基于固体表面润湿性理论，采用新型阳极氧化/喷砂酸蚀+碱热处理法制备具有微纳粗糙结构的超亲水性钛表面，然后采用双氧水处理等方法通过控制处理条件梯度去除钛表面羟基含量，从而构建出超亲-超疏梯度渐变润湿性的钛表面。然后，利用构建的模式表面，比较纤连蛋白在不同润湿性表面的吸附数量和构象变化，考察成骨细胞的黏附形态和分化能力对润湿性梯度变化的响应，评价不同润湿度的种植体在新西兰大白兔体内的骨整合情况。通过上述研究，阐明钛表面超亲-超疏梯度变化润湿性对其生物学效应的影响规律，进而探讨种植体的最佳润湿性参数范围，以期为钛种植体表面的综合优化设计提供科学依据。

The successful application of titanium dental implants in clinic with superhydrophilic surfaces demonstrates that it is an efficient strategy to endow implants with appropriate surface wettability for enhanced osseointegration ability. However, the biologic effect of titanium implants with wettability gradient is hardly elucidated, and the optimal wettability parameter remains unclear. Hence, we first aim at the methodology development to prepare titanium surfaces with wettability gradients. Based on the wettability theory of solid surface, we first prepare titanium surface with micro/nano hierarchical structures and superhydrophilicity using novel anodic oxidation/sandblast and acid etching+alkali heat treatment. Then, hydrogen peroxide solution treatment or other methods are applied to treat superhydrophilic titanium surface to remove the surface hydroxyl (OH) groups coverage gradually by controlling the treatment conditions. Eventually, a series titanium surfaces with wettablity gradients from superhydrophilicity to superhydropholicity could be fabricated. Moreover, they will be used as a research model for further investigation. The influences of wettability gradient on fibronectin adhesion and conformation will be compared, and osteoblastic adhesion and differentiation on titanium surfaces with different wettability was evaluated. In addition, the early osteointegration ability of titanium implants with different wettablity will be examined. Through the studies above, the effect of wettablity gradient that spans the entire range on the biologic performance of titanium will be revealed. Then optimal wettability range of dental implant will be explored, which may provide scientific evidence for optimum design of titanium implants surface.