松香基手性高分子微球的合成及手性识别机理研究

松香是一种天然可再生资源，其手性三环二萜结构不仅具有一定的刚性，而且具有良好的手性分离与识别性能。针对现有高分子型手性固定相机械强度低、重复使用性差的缺陷。本项目以松香为原料，经加成、酯化等反应合成松香基手性聚合单体，通过膜乳化-悬浮聚合法，利用自由基共聚、表面修饰及表面原位定向聚合接枝技术，合成松香基手性高分子微球，研究优化合成条件控制手性识别功能基密度、孔径和机械强度。以黄烷酮类手性化合物为拆分对象，采用高效液相色谱法，系统评价松香基手性高分子微球的手性识别性能；通过色谱热力学及吸附微量热力学，研究手性识别过程中的驱动力，结合现代仪器分析探索手性识别过程中分子立体结构变化规律，揭示松香基手性高分子微球对黄烷酮类对映体的手性识别机理。本项目成果将为合成机械强度高、手性识别能力强、绿色安全的松香基手性固定相提供新的思路和方法，也为松香基手性固定相应用提供理论依据和实验基础。

Rosin is one of the natural renewable resources, and its chiral tricyclic diterpene structure not only show a certain rigidity, but also is good for chiral separation and recognition. To overcome the low mechanical strength and poor reusability of existing polymer type chiral stationary phases, this project will investigate the synthesis of rosin-based chiral polymer microspheres by means of membrane emulsification suspension polymerization via free radical copolymerization, surface modification and in situ graft polymerization technology, with rosin-based chiral monomer as starting material which will be prepared through addition, esterification reaction of rosin, the functional-group density, pore size and mechanical strength will be controlled by optimizing the synthesis conditions. With flavanone enantiomers for chiral resolution, high performance liquid chromatography will be used to evaluate the chiral recognition performance of rosin-based chiral polymer microspheres. The driving force of chiral recognition will be studied by means of chromatographic thermodynamics and adsorption thermodynamics. Combined with the modern instrument analysis technology, changes of three-dimensional molecular structure in the process of recognition of chirality will be explored, and the chiral recognition mechanism on flavanone enantiomers will be revealed. This project will not only provide new ideas and methods for the synthesis of rosin-based chiral stationary phase with high mechanical strength, strong chiral recognition ability and green safety, but also provide a theoretical and experimental basis for the use of rosin-based chiral stationary phase.