载重组釉原蛋白纳米水凝胶的制备及其在牙周再生中的实验研究

在牙周组织再生的研究中，作为釉基质蛋白(eamel matrix proteins, EMPs)主要成分的釉原蛋白(amelogenin, Am)被认为是能诱导牙周组织再生的有效生物活性因子之一。但体内直接使用生长因子，会很快被稀释代谢或酶的作用而失活。近年来将纳米系统引入活性生长因子的缓释，为外源性生长因子的开发与利用开辟了新的研究空间。我们前期研究表明重组釉原蛋白能作用并影响牙周相关细胞的生物学功能，从而促进牙周组织再生。因此，本课题将在前期研究基础上，比较观察重组人釉原蛋白对牙周膜成纤维细胞生物学特性的影响；并制备纳米级的重组釉原蛋白-水凝胶纳米缓释系统，初步进行其安全性评价和动物实验研究，为牙周再生医学和组织工程领域外源性生长因子的开发和临床应用进一步提供理论基础和实验依据。

In the studies of periodontal regeneration, amelogenin (Am), which is the main biological active component of enamel matrix proteins (EMPs), was found to be capable of inducing periodontal tissue regeneration. However, growth factors will soon be inactive by metabolism or the action of enzyme when they were used directly in vivo. In recent years, the nanometer system was used for the release of active growth factors, which opened up a new field for the development and utilization of exogenous growth factors. Our previous studies have shown that the recombinant amelogenin (rhAm) can have biological effects on periodontal cell so that it may promote the regeneration of periodontal tissues. Based on the previous studies, this study is to observe comparatively the biological effects of hrAm on human periodontal ligament cells, to prepare the nanometer-sized rAm-hydrogel drug delivery system, and to investigate preliminarily the safety and function of the system through animal experiments. This study will further provide theoretical basis and experimental data for the development and clinical application of exogenous growth factors in the field of periodontal tissue engineering.