通过表面改性技术加快钛植入体早期骨整合修复进程是当前医用金属材料改性研究的重要手段。研究表明，在骨生长与再生过程中成骨化与成血管化耦合发生，因此研发具有促成骨化与促血管化作用的骨修复材料已成为骨再生医学领域研究的热点方向。本项目拟采用层层组装技术，利用装载匹伐他汀的β环糊精接枝壳聚糖和明胶在钛材表面构建含匹伐他汀涂层膜结构，研究多层膜结构在体外条件下对间充质干细胞的促成骨分化作用和对内皮细胞的促血管化作用，以及在大鼠皮下移植和骨缺损移植模型中对钛材表面-组织促骨再生的修复作用，重点探究含匹伐他汀涂层对促成骨化和促血管化交互作用的影响规律，揭示其内在分子机制。本研究不仅可进一步完善钛材表面改性策略设计及制作理论，同时可为其临床应用提供实验研究基础，具有重要的科学意义和应用价值。

Surface modifications of titanium implant to facilitate its early osseointegration is an important method in the field of metallic biomaterials modification research. Recent studies indicated that the growth of blood vessels in bone and osteogenesis are coupled during bone growth and regeneration process, and therefore development of bone repair biomaterials with enhanced angiogenesis and osteogenesis effects has becomes a hot research topic in the field of bone regeneration medicine. In this project, the pitavastatin loaded β-cyclodextrin grafted chitosan and gelatin are used as polyelectrolytes to construct a pitavastatin coating film structure on titanium substrates via a layer-by-layer (LBL) assembly technique. Promoted effects of multilayer structure on osteogenic differentiation of mesenchymal stem cells and angiogenesis of endothelial cells, as well as bone regeneration and repair in the rat subcutaneous and bone defect transplantation model are investigated. In particular, the regulation effects of pitavastatin containing film coated titanium substrates on the cell-cell interaction between angiogenesis and osteogenesis, and the underlying molecular mechanism will be further studied. The findings will not only improve the theory for design and fabrication of titanium surface modification strategy, but also provide a basis research for its clinical application.