当昆虫取食植物时，多数植物能够通过识别昆虫唾液中的某些小分子物质或蛋白产生特异的抗虫响应，比如鳞翅目昆虫唾液中的FAC（fatty acid-amino acid conjugate）类诱导物。虽然对FAC诱导的抗虫响应已做了较深入的研究，但是植物如何识别FAC还很不清楚。我们发现栽培番茄不能对昆虫唾液中的FAC成分产生响应，而野生番茄能够特异地响应FAC成分。利用野生番茄/栽培番茄衍生的F2群体，我们在第3染色体定位到一个能响应昆虫唾液诱导物FAC的QTL，我们将它命名为RTF。本申请将在此基础上，利用F2大群体的极端隐性单株，采用图位克隆的方法，同时结合第二代测序技术，将RTF精细定位到30kb。同时利用转基因完成功能互补验证，确证分离该基因。利用分子生物学技术，鉴定上下游调控基因，揭示RTF响应昆虫唾液分泌物FAC的调控机理,将对植物与昆虫之间相互关系的研究具有重要意义。

When insects feed on plants, most plants are able to deploy specific defense against insect attack by perceiving some small molecules and proteins, such as the elicitors FAC (fatty acid-amino acid conjugate) in Lepidoptera oral secretions. Although the FAC-induced defense responses have been intensively studied, yet little is known about how plants perceive FAC. Previously we found that the cultivated tomato Solanum lycopersicum does not perceive FAC in insect oral secretions, while the wild tomato S. pennelli is able to perceive FAC specially. One QTL involved in perceiving to FAC was mapped on chromosome 3 using a F2 population derived from the cross between wild tomato and cultivated tomato. Here we name the QTL as RTF (response to FAC). RTF can induce the synthesis of jasmonic acid in plants, and is a dominant gene. In this project, we will plant a large F2 population and select extreme recessive plants with insensitivity to FAC and use the next-generation sequencing technology to assist fine-mapping of RTF. In order to confirm the identity of RTF, wild tomato alleles of the candidate genes will be introduced into cultivated tomato by Agrobacterium-mediated transformation. We will discover its spatial and temporal expression patterns, identify its up-stream and down-stream genes, and unveil the mechanism underlying how this QTL is involved in perceiving herbivore-derived FAC signals using multiple approaches. Our goal is to establish the gene regulatory network in plant perception of herbivore-derived signals, which involves RTF, and to comprehensively understand how plants perceive herbivore-derived signals. Cloning and characterization of RTF will facilitate our understanding of plant-insect interactions.