伴随着我国深海取样装备技术的发展，获取6,000 m以下至11,000 m的深渊样品成为可能，但分离超嗜压微生物仍然是国际性的难题。前期我们提出了微生物在不同极端环境下有共同适应机制的假说，据此我们利用带有离散剂且成分确定的培养基，富集培养出能在152 MPa下快速生长的嗜冷超嗜压微生物，打破了已知嗜冷嗜压微生物的最适（98 MPa）、最高（140 MPa）生长极限。本研究中力求实现三个目标：1. 超高压条件下加离散剂和未加离散剂培养的微生物代谢途径的差异；2. 分离嗜冷严格超嗜压菌；3. 进一步打破低温条件下微生物生长的高压生长边界(>152MPa)。

With the current development of sampling technology, more and more samples were collected from hadal zone which is at the water depth between 6,000 m to 11,000 m during Chinese cruises. However, it is still an apparent challenge to isolate hyperpiezophiles. Previously, we have proposed that “the common adaptation strategy allows microorganisms adapting to high-hydrostatic pressure similar as to other environmental stresses”. Following this approach，we recently have enriched the psychrophilic and hyperpiezophilic strains at 152 MPa with defined medium supplemented with chaotrope, which broken the up-limit of the optimal-growth-pressure (98 MPa)and the highest-growth-pressure (140 MPa)for piezophiles. In this project, we are achieving three goals: firstly, analyze the microbial metabolic pathways and identify the difference between the enrichment cultures with and without chaotrope under extreme high pressure; secondly, isolate the psychrophilic and hyperpiezophilic strains; thirdly, raise the pressure boundary for microbial growth(>152MPa).