性能优良的香豆素-半花菁结构的荧光探针被用于细胞内小分子和离子的检测，但是因为分子结构的复杂性，关于其激发态动力学的机理并不清楚。本项目设计合成具有不同结构变化规律的BOB系列荧光探针，研究分子内可能存在的激发态能量转移，电荷转移以及异构化过程，揭示BOB系列荧光探针分子结构相关的分子内生色团相互作用的机理，并总结这一类荧光探针分子对不同应用背景（微粘度探测，极性环境探测，H2S等传感应用）的光谱变化特征。研究结果对于制备和合理利用这种功能化优异的荧光探针分子有重要的指导作用，有助于其在生物标记、细胞检测等方面的广泛应用，对改进和发展新型的荧光探针分子提供启发。

The coumarin-hemicyanine fluorescent probes with remarkable properties have been used to detect intracellular small molecules and ions, however, the excited state dynamics processes are still unclear because of their complex molecular structures. In this project, a series of coumarin-hemicyanine (BOB) fluorescent probes will be designed and synthesized with various structures, in order to study the intramolecular energy transfer, charge transfer and photo-isomerization processes. The present research will reveal molecular structure dependent intramolecular interaction mechanisms between chromophores within these fluorescent probes and summarize spectral features for different applications, e.g. high sensitivity micro-viscosity or polarity detection etc. Our results will play an important role in understanding of the photophysical properties of the functionalized fluorescent probes and stimulate their wide applications in biolabeling and cell detection. In addition, this work will provide inspiration for the improvement and development of novel fluorescent probes.