松墨天牛肠道噬纤维细菌Siphonobacter aquaeclarae 木质纤维素降解机制及其对宿主个体发育的响应

肠道细菌能够与植食性昆虫协同降解和消化木质纤维素，在共同应对昆虫个体发育进程中生境和食源的变化中发挥了重要作用。研究昆虫肠道细菌的木质纤维素降解功能，有助于深入认识肠道细菌与昆虫的关联和互作机制。申请人前期研究发现，松墨天牛肠道木质纤维素降解优势菌噬纤维细菌Siphonobacter aquaeclarae，是蛀干害虫肠道功能细菌的一个新成员。本项目拟研究S. aquaeclarae对木质纤维素各组分的降解功能和作用机制，探究其响应松墨天牛个体发育进程中的种群动态分布和酶解关键基因表达变化，明确接种S. aquaeclarae对松墨天牛生长发育、繁殖力和存活率的影响，揭示S. aquaeclarae木质纤维降解机制及其对宿主松墨天牛个体发育的响应。研究结果有助于深入认识松墨天牛的营养代谢和对食物的适应性，理解肠道细菌与松墨天牛之间的互作机制，为寻求调控松墨天牛种群的新方法奠定基础。

Intestinal bacteria play an important role in coordination with phytophagous insect to digest lignocelluloses, which is benefit for host insect in adapting to changes of environment and diet. To know more about the relationship between gut bacteria and insect, we need study on ligocellulose degradation of insect gut bacteria, In the previous studies, Siphonobacter aquaeclarae, a bacterium with the highest cellulose degradation ability in Monochamus alternatus gut, was isolated from woodboring insect for the first time. This project will research the ligocellulose degradation mechanism of S. aquaeclarae; clarify its population dynamic and the expression difference of key enzymes during the ontogenesis of M. alternatus; illuminate the influence to the development, reproduction and mortality of host by feeding S. aquaeclarae; reveal ligocellulose degradation mechanisms of S. aquaeclarae and the response to ontogeny of M.alternatus. The result will help to understand the nutrition metabolism and habitat adaption of M. alternatus, and lay the foundation for a new approach to control M. alternatus.