配子体自交不亲和机制是柑橘类果实无籽的重要机理之一。在植物配子体自交不亲和反应中，SCF复合体是影响授粉后是否亲和的关键决定因子，大量研究证实在茄科植物和苹果中SCF复合体介导了配子体自交不亲和反应中的泛素化降解途径，但在柑橘中对于SCF复合体参与泛素化途径的机制研究鲜有报道。申请者前期已克隆了香水柠檬S-RNase同源基因和泛素化途径关键因子SCF复合体组份候选基因，推测香水柠檬中可能存在以SCF复合体主导的泛素降解途径。本项目在前期研究基础上，探索SCF复合体组分的互作关系，利用泛素化蛋白组学分析和Western blot实验，从体内和体外模拟共同验证SCF复合体对香水柠檬S-RNase蛋白的泛素化标记。项目实施可进一步探明香水柠檬配子体自交不亲和机制，完善柑橘类果树自交不亲和模型，为探明柑橘类果树自交不亲和机理和柠檬无籽育种提供理论依据。

Gametophyte self-incompatibility (GSI) is one of the important mechanisms of seedless in citrus . In the plant gametophytic self-incompatibility, SCF complex is a key determinant of compatibility or incompatibility after pollination. Although the ubiquitin degradation pathway mediated by SCF complex was confirmed in Solanaceae plants and apples, there is no report about the ubiquitin at degradation pathway of S-RNase by SCF complex in citrus. In our previous work, we have cloned S-RNase homologous gene, part genes of SCF complex from ‘Xiangshui’lemon. Thus, the ubiquitin degradation pathway led by SCF complex is likely to exist in ‘Xiangshui’ lemon. On the basis of previous studies, we study the interaction of components of SCF complex, later we simulated S-RNase to be ubiquitination labeled and the SCF complex led the ubiquitination of S-RNase in vivo and vitro by analysis of ubiquitochemical proteins histological and western blot. The implementation of the project can further explore the mechanism of gametophyte self- incompatibility of ‘Xiangshui’ lemon, improve the lemon self-incompatibility model, explore the mechanism of citrus in self-incompatibility, and provide a theoretical basis for seedless breeding of lemon.