山豆根多糖对PCV2感染免疫细胞NF-κB炎症信号通路相关LncRNA的调控作用及其分子机制

本项目拟选择小鼠、猪的免疫细胞为研究对象，以免疫药理学理论为基础，结合分子生物学新技术，探讨山豆根多糖对病毒感染免疫细胞NF-κB炎症信号通路相关LncRNA的调节作用及其分子机制。分离提取及纯化山豆根多糖；采用猪圆环病毒-Ⅱ型体内外感染免疫细胞，通过体内外实验观察病毒感染对免疫细胞内炎性因子水平影响及山豆根多糖的调节作用；从NF-κB炎症信号通路相关LncRNA差异表达方面，观察LncRNA在病毒感染免疫细胞炎性反应中的作用；从LncRNA表达、炎性因子水平及其相关基因表达和蛋白表达方面，观察多糖对病毒感染免疫细胞NF-κB炎症信号通路相关LncRNA的调节作用；从LncRNA-X定位调控的靶基因表达方面，探讨多糖对病毒感染免疫细胞NF-κB炎症信号通路相关LncRNA调控机制,从而明确生物活性多糖调控炎性反应的分子机理，可为发现多糖药物作用靶点提供依据，具有重要学术意义和潜在应用价值。

The objective of this project is to investigate the regulatory effect and its molecular mechanism of active polysaccharides isolated from the roots of Sophora subprosrate on LncRNA related with NF-κB inflammatory signal pathway in PCV2 infected immunocytes of mice and pigs in vivo and in vitro. The study involves an intersection field of new technology of molecular biology and basic theory of immunopharmacology. In this project, regulatory effect of Sophora subprosrate polysaccharide on levels of inflammatory factors will be observed in vivo and in vitro in immunocytes infected by PCV2. The actions of LncRNA on the inflammatory reaction related with NF-κB signal pathway will be investigated by measuring differential expression of LncRNA in immunocytes infected by PCV2.The regulatory effect of the polysaccharide on LncRNA related with NF-κB signal pathway in PCV2 infected immunocytes in vivo and in vitro will be observed by investigating production of LncRNA, levels and gene expression as well as protein expression of inflammatory factors. The modulatory mechanism of the polysaccharide on LncRNA related with NF-κB inflammatory signal pathway will be studied by observing expression of target genes of LncRNA-X in the immunocytes infected by the virus and regulatory molecular mechanisms of active polysaccharides will be elucidated. The achievements will be of benefit to find a new drug target of polysaccharides and control virus infections in animals.