Whole Transcriptome Analysis of Intervention Effect of Sophora subprostrate Polysaccharide on Inflammation in PCV2 Infected Murine Splenic Lymphocytes.
Whole Transcriptome Analysis of Intervention Effect of Sophora subprostrate Polysaccharide on Inflammation in PCV2 Infected Murine Splenic Lymphocytes.
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DOI：
10.3390/cimb45070383
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发表时间：
2023-07-20
影响因子：
3.1
通讯作者：
Hu, Tingjun
中科院分区：
文献类型：
--
作者：
Zhao, Yi;Jia, Nina;Xie, Xiaodong;Chen, Qi;Hu, Tingjun
关键词：
(1)背景：槐花，为槐豆科植物槐Sophora tonkinensis Gagnep的干燥根及根茎。槐花多糖（SSP1）是从槐花中提取的，具有良好的抗炎和抗氧化作用。既往研究表明SSP1可以调节猪圆环病毒2型（PCV2）诱导的小鼠脾淋巴细胞炎症损伤，但具体调节机制尚不清楚。 (2)方法：采用全转录组分析来表征PCV2感染细胞和SSP1处理感染细胞中差异表达的mRNA、lncRNA和miRNA。利用基因本体论（GO）、京都基因与基因组百科全书（KEGG）等分析筛选与炎症相关的关键差异表达基因。通过RT-qPCR验证测序结果，并通过Western blot验证主要富集信号通路中的关键蛋白。 (3)结果：SSP1能够调节PCV2诱导的炎症相关基因变化，其干预机制主要涉及关键差异miRNA miR-7032-y、miR-328-y、miR-484-z。这些炎症相关基因主要富集于TNF信号通路和NF-κB信号通路，SSP1能够显着抑制p-IκB、p-p65、TNF-α、IRF1、GBP2和p-SAMHD1的蛋白表达水平，从而减轻炎症损伤。 （4）结论：从全转录组角度探讨了SSP1调节PCV2诱导的小鼠脾淋巴细胞炎症的机制，为SSP1的实际应用提供了理论基础。
(1) Background: Sophora subprostrate, is the dried root and rhizome of Sophora tonkinensis Gagnep. Sophora subprostrate polysaccharide (SSP1) was extracted from Sophora subprostrate, which has shown good anti-inflammatory and antioxidant effects. Previous studies showed SSP1 could modulate inflammatory damage induced by porcine circovirus type 2 (PCV2) in murine splenic lymphocytes, but the specific regulatory mechanism is unclear. (2) Methods: Whole transcriptome analysis was used to characterize the differentially expressed mRNA, lncRNA, and miRNA in PCV2-infected cells and SSP1-treated infected cells. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and other analyses were used to screen for key inflammation-related differentially expressed genes. The sequencing results were verified by RT-qPCR, and western blot was used to verify the key protein in main enriched signal pathways. (3) Results: SSP1 can regulate inflammation-related gene changes induced by PCV2, and its interventional mechanism is mainly involved in the key differential miRNA including miR-7032-y, miR-328-y, and miR-484-z. These inflammation-related genes were mainly enriched in the TNF signal pathway and NF-κB signal pathway, and SSP1 could significantly inhibit the protein expression levels of p-IκB, p-p65, TNF-α, IRF1, GBP2 and p-SAMHD1 to alleviate inflammatory damage. (4) Conclusions: The mechanism of SSP1 regulating PCV2-induced murine splenic lymphocyte inflammation was explored from a whole transcriptome perspective, which provides a theoretical basis for the practical application of SSP1.
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影响因子：
25.7
作者：
Navarro-Corcuera A;Sehrawat TS;Jalan-Sakrikar N;Gibbons HR;Pirius NE;Khanal S;Hamdan FH;Aseem SO;Cao S;Banales JM;Kang N;Faubion WA;LaRusso NF;Shah VH;Huebert RC
通讯作者：
Huebert RC
影响因子：
4.3
作者：
Ayuthaya, Benjar Issaranggun Na;Everts, Vincent;Pavasant, Prasit
通讯作者：
Pavasant, Prasit
影响因子：
1.8
作者：
Han J;Zhang S;Zhang Y;Chen M;Lv Y
通讯作者：
Lv Y
影响因子：
5.6
作者：
Chu, Yan-Biao;Li, Jun;Zhang, Shi
通讯作者：
Zhang, Shi
影响因子：
6.1
作者：
Li, Yuan;Miao, Miao;Guo, Shou-Dong
通讯作者：
Guo, Shou-Dong