纳米粒子载体的低组织靶向性和定域传输体系的低胞内转运效率是制约肿瘤治疗平台效能的关键问题。本项目提出以负载透明质酸酶（HAase）的柔性生物活性玻璃纤维（BGH）为组织定向输运体，以单宁酸（TA）/Fe3+层包覆的葡萄糖氧化酶（GOx）纳米粒子（GTF）为治疗因子，通过自组装构建复合纤维定域肿瘤协同治疗平台（GTF@BGH）。揭示湿化学电纺合成及热处理工艺等对柔性生物活性玻璃纤维的微结构、机械性能、降解性能等的作用规律，探明纤维微结构对透明质酸酶负载性能的影响机制；研究反应参数对GTF粒子物相的影响规律，探明其微结构对葡萄糖氧化酶和Fe3+负载的作用机制；阐明GTF@BGH复合纤维材料的粒子脱附和胞内转运行为，并揭示该复合纤维的体内外肿瘤治疗效能；从而获得一类兼具高组织定向传输、高效胞内转运、响应性治疗因子释放的饥饿/化学动力协同肿瘤治疗体系。

Particulate drug carriers (DDS) suffer low targeting delivery to disease tissue, while localized drug delivery systems (LDDS) present poor capability in the intracellular delivery of therapeutic factors. Both challenges significantly suppress the efficiency for cancer treatment via current therapeutic systems. We hereby propose a study for constructing a composite fiber therapeutic system, consisting hyaluronidase (HAase) loaded bioactive glass fibers (BGH) and tannic acid (TA)/ Fe3+ encapsulated glucose oxidase (GOx) nanoparticles (GTF) , aiming at combining the advantages of current LDDSs and DDSs. The influence of electrospinning process and heat treatment on the microstructure, mechanical property, and degradation property of flexible bioactive glass fibers will be investigated, and the relationship between microstructure of fibers and loading property of hyaluronidase will be illustrated; The influence of synthesis parameters on structure of GTF nanoparticles, and the relationship between microstructure of nanoparticles and loading properties of GOx and Fe3+ will be uncovered. Furthermore, the de-assembly and cell up-taking effect of GTF and anti-cancer properties of GTF@BGH composites will be systematically revealed. It is therefore anticipated this new type of therapeutic system, endowed with high tissue guiding, high cell up-taking and stimulus-triggered release of therapeutic factors, may provide a promising protocol in advanced starvation/chemodynamic synergistic therapy.