深海环境中噬菌体与宿主菌的相互作用十分活跃，噬菌体通过这种相互作用对海洋生态系统的食物链和物质循环发挥重要影响。虽然有越来越多的研究关注于深海噬菌体与宿主菌之间的蛋白互作，但是到目前为止国内外对噬菌体与宿主在代谢水平的相互作用却知之甚少，因此本项目拟在代谢水平上对代谢物质在深海噬菌体与宿主菌互作过程中的作用展开研究。在前期的工作中, 我们对一株分离自东太平洋深海热液口的嗜热噬菌体GVE2与其宿主菌Geobacillus sp. E263的相互作用开展了大量研究, 并筛选到了24种在噬菌体感染后显著上调或下调的宿主代谢物。在本项目中，我们重点研究这些噬菌体感染相关代谢物质对噬菌体侵染、裂解宿主的影响，并选择活性代谢物质进一步研究其发挥作用的具体通路，由此来揭示深海噬菌体与宿主在代谢水平的相互作用，为全面探索噬菌体在深海生态系统中的生态功能提供重要依据。

In deep-sea environments, bacteriophages interact with their hosts extensively. They have a significant impact on the food chain and biological matter cycle of deep-sea ecosystems by interacting with bacterial hosts. So far, considerable advances have been made in revealing the proteinic interactions between deep-sea bacteriophages and hosts, however, to our best knowledge, little is known about the role of metabolites in bacteriophage-host interactions. Thus, in our study the role of metabolites in bacteriophage-host interactions will be investigated in depth. In our previous studies, GVE2, a thermophilic bacteriophages isolated from the deep-sea hydrothermal vent in the east Pacific Ocean, had been extensively investigated on its interaction with Geobacillus sp. E263. A total of 24 metabolites were found to be significantly up-regulated or down-regulated during GVE2-host interaction. In this project, the role of phage-associated metabolites in the phage-host interactions as well as its action mechanism will be studied, which will help to elucidate the comprehensive effect of bacteriophages on the biosphere in deep-sea ecosystem.