EIN3互作蛋白EII6调控拟南芥叶片衰老启动的分子机理
结题报告
批准号:
31970303
项目类别:
面上项目
资助金额:
58.0 万元
负责人:
周欣
依托单位:
学科分类:
植物激素与生长调节物质
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
周欣
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中文摘要
乙烯是调控植物衰老的重要激素,但困扰科学界多年的叶片衰老启动的分子机理一直悬而未决。EIN3是乙烯调控衰老的关键转录因子,其复合体整合光和多种激素信号调节生长发育。为解析EIN3复合体在衰老启动期的功能,我们对复合体组分进行了分离,鉴定到EIN3 互作蛋白EII6。EII6是MADs 转录因子,乙烯处理后,eii6莲座叶在衰老窗口期之前呈叶片黄化表型,揭示EII6可能抑制叶片衰老启动。进一步研究发现EII6直接抑制乙烯合成酶基因ACS2和乙烯信号正调组分EIN2的转录,促进EIN3降解因子EBF1的表达。本项目拟在此基础上继续深入研究:EII6逐级调控乙烯合成、乙烯信号以及EIN3积累和蛋白活性的分子机理;继续分离衰老启动期EIN3复合体的新组分并解析其生物学功能;筛选EII6的增强子和抑制子。以期揭开EII6在植物生长发育早期全方位钳制乙烯途径,形成确保植物安全生长的窗口期的神秘面纱。
英文摘要
Ethylene is an important phytohormone that is crucial for modulating plant senescence. However, the underlying molecular mechanism involving the onset of leaf senescence still largely remains unresolved during the past decades. The transcription factor EIN3 or its associated protein complex not only plays a pivotal role in ethylene-mediated leaf senescence, but also functioning as a central regulatory element to regulate plant growth and development by integrating light and various other plant hormone signaling. To elucidate the precise function of EIN3 or its associated complex during the onset of leaf senescence, we purified and isolated a new component of the EIN3 associated protein complex, namely EII6 (EIN3 interacting protein6) which belongs to the family of MADs transcription factor. The loss-of-function mutant eii6 exhibits a yellowish rosette leaf phenotype prior to the narrow ‘senescence window’ stage upon ethylene treatment, indicating that the native EII6 protein may inhibit the onset of ethylene-mediated leaf senescence. We further showed that EII6 directly represses the transcription of ethylene biosynthetic gene ACS2 as well as the positive ethylene signaling component EIN2, whereas promotes the expression of EIN3 Binding F-box protein EBF1. This proposal will further focus on: 1) elucidating the molecular mechanisms of EII6 mediated regulation of ethylene biosynthesis, ethylene signaling, EIN3 accumulation as well as the activity of EIN3 protein; 2) identifying additional new components of the EIN3-associated complex during the onset of leaf senescence and analyzing their biological functions; 3) screening of EII6 enhancers and repressors. Our aim is to uncover the mystery of why EII6 could comprehensively suppress the entire ethylene pathway during the early stage of plant growth and development, hence forming the ‘senescence window’ stage to ensure healthy plant growing.
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DOI:10.1111/jipb.13075
发表时间:2021
期刊:Journal of Integrative Plant Biology
影响因子:11.4
作者:Wang Chaoqi;Dai Shouyi;Zhang Zhong-Lin;Lao Wenqing;Wang Ruiying;Meng Xianqing;Zhou Xin
通讯作者:Zhou Xin
DOI:10.1111/pce.13803
发表时间:2020-05
期刊:Plant, cell & environment
影响因子:--
作者:Zhen Yang;Chaoqiuyu Wang;Kai Qiu;Huiru Chen;Zhongpeng Li;Xin Li;Jiang-bo Song;Xiaolei Wang;Jiong Gao;B. Kuai;Xin Zhou
通讯作者:Zhen Yang;Chaoqiuyu Wang;Kai Qiu;Huiru Chen;Zhongpeng Li;Xin Li;Jiang-bo Song;Xiaolei Wang;Jiong Gao;B. Kuai;Xin Zhou
乙烯通过自噬途径促进植物叶片衰老的分子机制研究
  • 批准号:
    24ZR1456500
  • 项目类别:
    省市级项目
  • 资助金额:
    0.0万元
  • 批准年份:
    2024
  • 负责人:
    周欣
  • 依托单位:
国内基金
海外基金