国内外都在高度关注食品中氯丙醇酯污染及其安全限量问题。标准方法推荐水解氯丙醇酯后间接测定氯丙醇含量，存在操作繁琐/耗时、大量使用危险品、依赖大型仪器的不足，不能满足现场检测需要。脂肪酶水解-电化学分析具有快速、便捷、绿色的优势，但存在副反应很多，方法灵敏度低的缺陷。本项目制备一种脂肪酶/温控印迹介孔复合材料，一方面能选择性识别和富集酶解产物氯丙醇，降低样本基质和副反应产物干扰，提高方法灵敏度；另一方面，创新性引入DNA-化学双交联技术，借助DNA变性-复性实现印迹孔穴的可逆破坏-重建，解决传统印迹材料需要消耗大量溶剂长时间洗脱氯丙醇的弊端。通过系统研究印迹复合材料的制备及其机理，集成脂肪酶水解、分子印迹萃取和电化学分析技术，构建一种快速、绿色、灵敏的氯丙醇酯现场分析方法，为研发智能印迹材料及其食品安全“现场即时”检测技术提供研究思路，为制定或修订氯丙醇酯食品标准提供技术支持。

Domestic and foreign governments are paying close attentions to the pollutions of chloropropanol esters and their safety level in foods. The indirect method is proposed to quantify chloropropanol resulting from hydrolysis of chloropropanol esters. However, it requires expensive instruments, dangerous regents and time-consuming procedures and sometime not so suitable for the on-site detection. Enzymatic hydrolysis coupled with electrochemical analysis is a rapid, simple and green method, but interference of by-product and low sensitivity are technical problems. In this study, lipase/thermal-responsive imprinted materials using chloropropanol as the templates were prepared on mesoporous SiO2 microparticles. It can be applied to selectively recognize and enrich enzymolysis products to further improve the catalytic efficiency and the sensitivity. Furthermore, bis-acrylamide and nucleic acid duplexes as double cross-linkers are innovatively introduced to construct imprinting cavities. Based on the DNA denaturation and renaturation, these cavities can be destroyed or reconstructed to control the selectivity of imprinted composites. It can be used to solve the problems of common imprinting materials that chloropropanol has to be eluted with a lot of solvents. After studying the preparation and mechanism of imprinted composites, a fast, green and sensitive on-site analytical method is developed to determine chloropropanol ester in food samples. This study provides a novel ideas for preparation of smart imprinted materials and on-site detection, and then an approach to formulate or revise the food standards.