肿瘤相关标志物的检测对肿瘤临床诊疗具有重要意义。然而，目前临床上对肿瘤标志物(如肿瘤相关蛋白及外泌体)的检测方法存在灵敏度差、通量低、假阳性高等缺点。本项目从提高肿瘤临床诊疗效果的需求出发，拟构建一种集目标物准确识别及其信号转换、信号扩增与输出于一体的多探针共识别诱导的DNA自组装检测体系。在该体系中，以现有的固相免疫检测为基础，元素标记的DNA自组装部件作为信号分子和扩增部件。只有当所有DNA探针共同识别目标物时，才能诱导相关DNA进行自组装, 即有效减少假阳性。同时利用DNA扩增技术相关DNA部件进行信号扩增，再使用ICP-MS读取输出信号，实现在临床样本中对多个肿瘤相关标志物(肿瘤蛋白标志物及相关外泌体) 高精准、高灵敏和高通量的检测。最后将获得数据与临床数据做比对，确认本策略的临床实用价值，争取为改善肿瘤临床诊疗效果提供新的技术支持。

The analysis for tumor associated biomarkers is crucial for tumor theranostics. However, the current methods for tumor associated biomarkers (e.g. protein-based tumor biomarker and tumor-derived exosome) have the shortcomings, including poor sensitivity, low-throughputs, positive false and so on. To improve the tumor theranostics, this proposal intends to construct the recognition of multiple probes-induced DNA self-assembly system which integrates the functions of target recognition with high-fidelity, signal conversion and amplification. In this system, the capture antibody-loaded solid array is used as the analysis platform, the elements-labeled DNA self-assembly part is utilized as the signal molecule and amplification components. Only when all probes are recognized the target protein in a multiple manner, the relevant DNA self-assembly can be initiated, achieving the elimination of positive false. Incorporating with DNA amplification technique, the relevant DNA components can be amplified. Consequently, the ICP-MS are employed to yield the signal, achieving the multiple detection of tumor associated biomarkers with high-fidelity, high-throughput and high sensitivity. In confirmation with clinical data in the hospital, it is expected to this strategy will support to improve the tumor theranostics.