针刺是防治荨麻疹的传统疗法之一。肥大细胞（MCs）活化并脱颗粒释放炎性介质是荨麻疹发生的关键环节。以Lyn激酶为起始信号，IgE/FcεRI介导的PIP2/IP3/DAG信号通路是MCs脱颗粒的主要途径之一。前期研究提示，针刺曲池、足三里、三阴交可改善荨麻疹，抑制MCs脱颗粒和释放炎性介质，但具体机制未明。据此推测：针刺可能通过降低Lyn激酶活性，调节IgE/FcεRI介导的PIP2/IP3/DAG信号通路，抑制MCs脱颗粒，减少炎性介质释放，改善荨麻疹过敏症状。本项目拟采用免疫荧光、激光共聚焦、Western blot、ELISA、RT-PCR等技术，结合针刺干预荨麻疹模型大鼠和针刺血清干预脱颗粒模型RBL-2H3细胞实验，探讨针刺对PIP2/IP3/DAG信号通路关键基因和蛋白的调控作用，揭示针刺防治荨麻疹的科学内涵，为基础研究提供新方向和思路，为推广临床应用提供科学依据。

Acupuncture is one of the traditional remedies for urticaria.Mast cells (MCs) activation and degranulation induced inflammatory mediator release is the key mechanism of urticaria.With Lyn kinase as a starting signal, IgE / FcεRI-mediated PIP2/IP3/DAG signaling is one of the major pathways for MCs degranulation.Previous studies have shown that acupuncture Quchi, Zusanli and Sanyinjiao can improve urticaria, inhibit MCs degranulation and inflammatory mediators release, but the specific mechanism is unknown.It is speculated that acupuncture may reduce the Lyn kinase activity, regulate IgE-FcεRI mediated PIP2/IP3/DAG signaling pathway, inhibit MCs degranulation, reduce the release of inflammatory mediators and improve the symptoms of urticaria allergy.The project intends to apply immunofluorescence, laser confocal microscopy,Western blot, ELISA, RT-PCR technologies, combined with acupuncture intervention in urticaria model rats and acupuncture serum intervention in degranulation model RBL-2H3 cell experiments, to investigate the regulation effect of acupuncture on the key genes and proteins of PIP2/IP3/ DAG signaling pathway, reveal the scientific connotation of acupuncture prevention and treatment urticaria, provide new directions and ideas for basic research and scientific basis for the promotion of clinical application.