精准识别胶质瘤是其诊疗的关键。当前CT、MRI、肿瘤荧光等胶质瘤显影方法存在灵敏度偏低、特异性欠强及相互间整合互补难等问题。我们前期研究发现纳米载体碳量子点（CDs）具有自身荧光强、易通过血脑屏障等特性；同时胶质瘤细胞表面EGFRvIII可作为特异性靶点。据此，本研究设计以修饰MRI显影对比剂钆离子（Gd）的CDs为载体（Cds-PEG-Gd），连接特异性识别EGFRvIII的核酸适配体A32，构建可实现胶质瘤MRI增强/肿瘤荧光一体化双重显影的靶向探针CDs-PEG-Gd/A32；此外为了提高显影灵敏度，减少钆剂使用量，根据DNA组装编程原理，设计DNA序列构建辅助信号放大探针，并与靶向探针共同组建可实现信号放大的双重显影系统（DBPS）：通过靶向探针识别靶点来诱导辅助信号放大探针上DNA序列发生组装反应并形成探针聚集，从而实现信号发大，提高肿瘤显影灵敏度，更为精准指导胶质瘤手术定位。

Accurate identification of glioma is the key to its diagnosis and treatment. At present, CT, MRI, tumor fluorescence and other image detections have deficiencies such as low sensitivity, poor specificity and difficulty in integration and complementation. Our previous studies have found that nanocarrier carbon quantum dots (CDs) have strong self-fluorescence and easy to pass the blood-brain barrier. At the same time, EGFRvIII on glioma cells can be used as a specific target. Accordingly, this study is designed to modify the CDs of the MRI contrast agent gadolinium (Gd) as a carrier (Cds-PEG-Gd), and link the nucleic acid aptamer A32 that specifically recognizes EGFRvIII to construct MRI enhancement of glioma/ Tumor fluorescence integrated dual-imaging targeting probe CDs-PEG-Gd/A32; in addition, in order to improve the imaging sensitivity and reduce the amount of gadolinium, we designed DNA sequences to construct auxiliary signal amplification probes according to the principles of DNA assembly programming, and the targeting probes jointly form a dual imaging system (DBPS) which can achieve signal amplification: the DNA structure changes when the target probe recognizes the target,induces the assembly reaction of the DNA sequence on the DBPS and the auxiliary signal amplification probe, and the signal assisted detection Needle aggregation, to achieve signal amplification, improve tumor imaging sensitivity, and guide the glioma treatment more precisely.