间充质干细胞（MSCs）的成骨分化是骨缺损修复的重要过程之一。近期研究发现，物理刺激带来微环境的变化会影响干细胞分化方向，其作用可能与细胞骨架的变构、重组等有关。课题组前期研究研究显示，通过磁化涂层对细胞施加周期性力学刺激能促进干细胞的增殖和成骨分化，且不同方向磁场对应的细胞增殖情况有差异。在此基础上，本课题组拟对磁化胶原涂层的不同物理性征进行制备及表征；探究不同磁控三维力对细胞行为的影响，并从细胞骨架角度进一步阐明磁控三维力调控BMSCs成骨分化的机制；继而通过体内实验，评估经调控干细胞的体内生物学性能。本研究有望阐明磁控力对干细胞成骨分化的影响，同时在应用层面通过改良材料涂层来修复骨缺损，具有较高创新性与医学意义及潜在的应用前景。

Osteogenic differentiation of mesenchymal stem cells (MSCs) is one of the important processes of bone defect repair. Recent studies have found that physical stimulation is related to the growth behavior of stem cells, and its role may be related to the constitutive transformation and reorganization of the cytoskeleton. Previous researches by our group showed that periodic mechanical stimulation of cells by magnetized coating can promote stem cell proliferation and osteogenic differentiation, and the cell proliferation in different magnetic directions is different. On this basis, our group intends to prepare and characterize the different physical properties of magnetized collagen coatings, then explore the effects of different magnetron three-dimensional forces on cell behavior, and further clarify the mechanism of osteogenic differentiation of BMSCs under the control of the magnetron three-dimensional forces from the perspective of cytoskeleton. Moreover, in vivo experiments to assess the in vivo biological properties of the regulated stem cells shall be done. This study is expected to clarify the effect of magnetic control force on the osteogenic differentiation of stem cells, and to cure bone defects through improved material coating at the application level, which has high innovation and medical significance and potential application prospects.