我国丰富的野生大豆是大豆重要性状改良的潜在遗传资源，但其固有的蔓生特性限制了其在栽培大豆育种改良中的利用。利用栽培大豆中黄39与野生大豆NY27-38构建F2:3和RIL分离群体，在海南三亚种植呈现明显的直立与蔓生性状的分离。利用180K高通量SNP芯片检测蔓生和直立DNA池（BSA法），在大豆2号和19号染色体定位到与蔓生性相关的两个QTL位点，献率分别为5.3%和20.7%的。本项目拟对19号染色体的主效QTL进行精细定位，图位克隆蔓生相关基因；创建近等基因系材料，研究其受光温调节的生理特性及候选基因表达特点；利用遗传转化和转录组测序，验证基因的功能，阐明其调控蔓生形成的分子机制；通过对代表性野生和栽培大豆资源的基因序列分析，明确单倍型的变异特点和地理分布特征，开发功能基因分子标记，为利用野生大豆创新种质、改良大豆品种提供新思路。

Wild soybean accessions are potential genetic resources for cultivated soybean improvement in some specific agronomic traits, whereas the utilization efficiency of wild soybean was affected by the negative contribution of domestication related vining habit. The lines of F2:3 and RIL populations from Zhonghuang 39× NY27-38 (wild soybean) showed obvious variation of erect and vining habit when planted in Sanya, Hainan island. Two QTLs, which accounted for 5.3% and 20.7% phenotypic variation, associated with vining habit were detected on soybean chromosome 2 and 19 by testing the bulked DNA pools (vining and erect) with 180K Amiom SoyaSNP array to. Here, in this research , we will map-based clone the candidate gene on chromosome 19 through fine mapping approach, and explore the underling genetic mechanism by combing physiology character evaluation, transcriptome-wide RNA sequencing analysis of near isogenic lines. Functional validation of candidate gene will be done through genetic transformation. To better understand the variation of candidate gene, representative wild and cultivated soybean accessions will be sequenced at the candidate locus. Analysis of haplotypes and their geographical distribution will be performed to reveal the association of genomic variation and vining habit during soybean domestication. Functional molecular markers will be developed and used for evaluation of the haplotype. This research will provide novel point for effective use of wild soybean germplasm.