土壤盐渍化是影响大豆产量的重要非生物胁迫因子之一，前期研究发现在栽培大豆和野生大豆中都有耐盐资源。利用耐盐野生大豆ZYD3687与栽培大豆杂交构建了F2和BC1F2分离群体，初步分析发现野生大豆中存在不同于栽培大豆的耐盐基因。发掘大豆资源中不同的耐盐基因，对于通过基因累加培育耐盐大豆品种具有重要意义。本项目拟通过耐盐、盐敏感基因池转录组测序分析与群体定位相结合，快速确定耐盐基因所在的染色体区间。利用该区间功能基因的单核苷酸多态性（SNP）和插入/删除（INDEL）信息开发功能标记芯片，通过次级分离群定位及自然群体的关联分析，精细定位并克隆耐盐候选基因。分析野生大豆中候选基因的时空表达特性，通过发根农杆菌转化大豆及病毒介导的基因沉默技术验证候选基因的功能，分析候选基因的生理特点，揭示候选基因的耐盐机制，为通过栽培大豆与野生大豆耐盐基因累加的分子标记辅助育种提供理论依据。

Salinity is an important abiotic stress that affect soybean yield. We found salt tolerant germplasm in both cultivated soybean (Glycine max.)and wild soybean (Glycine soja.)in previous work. F2 and BC1F2 populations were created by crossing wild soybean ZYD3687 and cultivated soybean. We found there may has different salt tolerance gene in wild soybean by analyzing some individuals of the F2 population. Mapping and using salt tolerant genes in different soybean germplasm play important roles in pyramiding different tolerant genes during molecular design breeding. In this study, salt tolerant and sensitive gene pools will be made by evaluate the salt tolerance of the F2 population. A combination of genetic mapping and bulked transcriptome profiling will be used to rapidly map the salt tolerant gene in wild soybean. This will be an effective way to identify the candidate region of salt tolerance gene in soybean chromosome and find functional SNP and INDEL polymorphism within gene(s). These markers will be used to screen segregating populations and map the candidate gene by association mapping. The expression chatacters and function of candidate gene will be evaluated by over expression and virus induced gene silencing (VIGS) method using cucumber mosaic virus vectors. This will be suitable for developing functional markers for marker assisted breeding for soybean salt tolerance and help to make clear of the mechanism of gene regulation for salt tolerance in wild soybean.