拟南芥类受体胞质激酶PBL19介导几丁质信号转导与真菌抗性的机制研究

批准号:
31970278
项目类别:
面上项目
资助金额:
58.0 万元
负责人:
李剑峰
依托单位:
学科分类:
植物与环境互作
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
李剑峰
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中文摘要
病原真菌对植物生长造成严重威胁,但目前真菌引发的植物免疫机制远不如细菌清楚,这极大制约了植物抗真菌基因资源的挖掘以及抗真菌新策略的开发。我们在前期研究中发现,拟南芥类受体胞质激酶PBL19能够正调控对灰霉菌和核盘菌的抗性。真菌几丁质能够诱导共受体CERK1磷酸化PBL19以及PBL19从细胞膜向细胞核的迁移。持续入核的PBL19会产生植株矮小和组成型免疫的表型。该表型依赖于PBL19自身启动子及激酶活性,并受到高温抑制。RNA-seq分析表明PBL19组成型入核会导致PBL19自身以及水杨酸(SA)相关基因的表达上调,这与该植株中SA的大量积累吻合。本项目拟在上述研究基础上,阐明PBL19介导几丁质信号转导及真菌抗性的分子机制。本研究首次发现几丁质受体下游具有可发生膜核迁移的信号转导蛋白,将为研究真菌引发的植物免疫信号转导提供新的范式,并将揭示几丁质诱导植物SA合成及真菌抗性的新机制。
英文摘要
Fungal pathogens pose a severe threat to plant growth. However, the plant immunity against fungal pathogens is much less understood compared to that against bacterial pathogens, which greatly limits the discovery of new fungi-resistant genes and the development of novel antifungal strategies. Our preliminary data indicate that the Arabidopsis PBL19, a receptor-like cytoplasmic kinase, can positively regulate plant immunity against fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum. Fungal elicitor chitin can trigger PBL19 phosphorylation by the co-receptor CERK1 and subsequent relocation to the nucleus from the plasma membrane. Constitutive nuclear localization of PBL19 will cause dwarfism and constitutive immunity in plants, which, however, also requires the kinase activity of PBL19 and the native promoter for PBL19 expression. In addition, the phenotypes of dwarfism and constitutive immunity can be suppressed by higher temperatures. RNA-seq analysis reveals that constitutive nuclear localization of PBL19 will lead to substantial upregulation of its own gene and SA-related genes, which is consistent with the finding that SA is overaccumulated in the plants. Based on these preliminary data, we propose to study the role and regulatory mechanism of PBL19 in chitin signaling and fungal resistance. The proposed work will identify the first membrane-nuclear trafficking component in chitin signaling and uncover new mechanism underlying chitin-induced SA biosynthesis and fungal resistance in plants.
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DOI:--
发表时间:2020
期刊:Trends in Plant Science
影响因子:--
作者:Ben-Qiang Gong;Feng-Zhu Wang;Jian-Feng Li
通讯作者:Jian-Feng Li
DOI:10.1007/s44154-023-00110-4
发表时间:2023-07-22
期刊:Stress biology
影响因子:--
作者:
通讯作者:
Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity
受体样细胞质激酶的质膜-核-质协调促进EDS1依赖性植物免疫
DOI:10.1038/s41477-022-01195-x
发表时间:2022-07-18
期刊:NATURE PLANTS
影响因子:18
作者:Li, Yujia;Xue, Jiao;Li, Jian-Feng
通讯作者:Li, Jian-Feng
拟南芥天生免疫中GPA1调控细菌鞭毛信号转导的分子机制研究
- 批准号:31770295
- 项目类别:面上项目
- 资助金额:60.0万元
- 批准年份:2017
- 负责人:李剑峰
- 依托单位:
拟南芥CDG1负调控免疫信号转导中MAPK激活的分子机制研究
- 批准号:31570276
- 项目类别:面上项目
- 资助金额:63.0万元
- 批准年份:2015
- 负责人:李剑峰
- 依托单位:
国内基金
海外基金
