真菌毒素是一类存在广泛、危害巨大的食品污染物，由于污染范围广、去除难、限量低，所以开发针对真菌毒素的快速、稳定、灵敏的检测方法具有十分迫切的实际意义。本项目主要目标是在材料、流程与方式上对真菌毒素快速检测方法进行改善，进而提出Fe3O4-Pt纳米酶二维网络结构对真菌毒素快速便捷、高稳定性、高灵敏度的多重增敏检测新方向。解析Fe3O4-Pt核壳结构中元素协同作用机制，实现类过氧化物酶催化能力的高效提升；探究纳米酶免疫学修饰原理，验证磁分离富集净化效率水平，实现真菌毒素检测浓度的提高；衔接前处理与检测步骤，揭示二维网络结构组装机理，阐明空间协同效应作用规律，实现体系催化效率的提升。最终分析酶促反应光学信号强度与真菌毒素含量之间内在响应机制，明确多重协同增敏机理，确定检测方法实用性。本项目的研究可以建立针对真菌毒素的多重增敏检测体系，进而为真菌毒素快速、准确和灵敏的检测提供理论依据和实验基础。

Mycotoxins are widespread food contaminant with great harm. Because of the multiple types of pollution, difficult to remove, and the lower maximum limit, it is urgently to develop the rapid, stable, and highly sensitive method for mycotoxin detection. The aim of this study is to improve the determination method of mycotoxin in material, process and mode. So the nano-enzyme two-dimensional network structure composed of Fe3O4-Pt/Core-Shell nanoparticles was used to establish a multiple enhancement sensitivity method for mycotoxin detection with convenience, stability and sensitivity. The element synergism mechanism of Fe3O4-Pt/Core-Shell nanoparticles was analyzed to improve the catalytic capacity of materials like peroxidase. The detection concentration of mycotoxin was increased based on the exploration of immunological modification principle and verification of immunoaffinity enrichment ability. The modified nano-enzyme was used to connect pre-processing and detection process. Then the improvement of the system catalytic efficiency was based on the revelation of two-dimensional network structure assembly mechanism, and clarification of spatial synergistic effect. Finally, the intrinsic response mechanism between the optical signal strength of enzymatic reaction and the content of mycotoxin was analyzed, and the mechanism of multiple enhancement sensitivity was clarified. The practicability of the established method was determined. This research of the project can be used for the establishment of a multi-sensitization detection system for mycotoxin, and to provide theoretical basis and experimental basis for rapid, accurate and sensitive detection of mycotoxin in complex food matrix.