重离子是肺癌放疗的优选射线，但辐射旁效应（RIBE）可导致肺癌微环境重要间质细胞BMSCs基因组损伤而影响其临床应用。基因芯片结果提示可能与TNF-α/HIF-1α/iNOS有关，但未充分证实。中医学认为辐射可耗气伤津、病及精元，通过脏腑互传导致BMSCs精气虚损，营卫失调。课题组前期证明有益气补虚功效的黄芪防护效果明显但物质基础未明。本研究拟通过细胞实验研究重离子辐射肺癌相关BMSCs基因组损伤中TNF-α、HIF-1α和iNOS的分子机制；借助分子对接虚拟筛选能与关键靶蛋白(TNF-α、HIF-1α、iNOS)结合的黄芪小分子；微量热泳动及细胞实验筛选黄芪小分子最佳配伍并进一步从动物、细胞、分子水平探究其对重离子辐射肺癌致BMSCs基因组损伤的保护作用，揭示黄芪多点显效、协同增效防护重离子辐射肺癌相关BMSCs基因组损伤的物质基础及分子机制，为中医药联合辐射治疗肺癌提供理论依据。

Heavy ions are the preferred radiation for lung cancer radiotherapy, but radiation-induced bystander effect (RIBE) can cause damage to the genome of bone marrow mesenchymal stem cells (BMSCs), an important interstitial cell in the lung cancer microenvironment, and affect its clinical application. Gene chip results suggest that it may be related to TNF-α/HIF-1α/iNOS signaling pathway, but it has not been fully confirmed. According to traditional Chinese medicine, radiation can consume Qi, injure body fluid, disease and essence, and lead to deficiency of essence and Qi and maladjustment of camp and health of BMSCs through mutual transmission of viscera. At the early stage of our research group, it has been proved that the protective effect of Astragalus membranaceus is obvious, but the material basis is not clear.This study intends to study the molecular mechanism of TNF-α, HIF-1α and iNOS in the genome injury of BMSCs associated with heavy ion radiation lung cancer through cell experiments.Virtual screening of effective Astragalus molecules that can bind to key target proteins (TNF-α, HIF-1α, iNOS) through molecular docking. Microthermophoresis and cell experiments screened the best compatibility of Astragalus small molecules and further explored its protective effect on the BMSCs genome damage caused by heavy ion radiation lung cancer from animal, cellular and molecular levels and to reveal the material basis and molecular mechanism of multi-point and synergistic protection of Astragalus membranaceus membranaceus against the genomic damage of BMSCs related to heavy ion radiation lung cancer, so as to provide a reference for the treatment of lung cancer by combination of traditional Chinese Medicine.