石斑鱼是我国南方及东南亚各国重要的海水名贵养殖鱼类之一，病害频发严重影响其可持续健康发展，石斑鱼虹彩病毒（Singapore grouper iridovirus，SGIV）是重要的传染性病原之一。我们的前期研究表明，网格蛋白介导的内吞（clathrin mediated endocytosis，CME）是SGIV侵染宿主细胞的重要途径之一。网格蛋白（clathrin）作为CME的标志性蛋白对病毒侵染有重要作用。然而，目前有关SGIV如何利用clathrin侵染宿主细胞的精细作用机制仍不十分清楚。因此，本项目拟克隆石斑鱼clathrin基因，通过多种技术研究clathrin对SGIV侵染宿主细胞的影响，及二者的动态作用过程，并初步鉴定与clathrin相互作用蛋白，从而揭示clathrin介导SGIV侵染宿主细胞的机制。研究结果将进一步阐明虹彩病毒的致病机理，并促进发展新的抗病毒策略。

Grouper, Epinepheus spp., is among the most important marine-cultivated fishes in the southern coastal areas of China and Southeast Asia, but viruses, such as Singapore grouper iridovirus (SGIV), one of the most important viral pathogens in culturing groupers and other marine fishes, have already highly threatened the health of the fish thus the sustainable development of grouper aquaculture. Based on the previous studies, clathrin- mediated endocytosis (CME) is crucial in SGIV entry, and as the marker protein of CME, clathrin plays vital role in virus infection. However, the detailed mechanisms how SGIV infects host cells through CME remain largely unknown. To reveal the mechanisms of clathrin mediated SGIV entry and then to improve antiviral drug design, we will in this study clone the grouper clathrin gene, take advantage of combined technologies to study the effect of clathrin on SGIV infection, investigate the dynamic interactions between SGIV and clathrin in living cells and in the end identify protein complex of clathrin.