土壤盐渍化已成为世界农业生产面临的主要威胁之一。鉴于传统作物耐盐性普遍较差，从极端盐生植物中发掘耐盐基因，培育耐盐作物新品种是解决土壤盐渍化问题的有效途径。盐生草（Halogeton glomeratus）是一种广泛分布于我国西北旱区的肉质化盐生植物资源。本项目拟以盐生草中克隆到的耐盐新基因HgS2为对象，从解析该基因在盐生草叶肉细胞Na+液泡区隔化转运过程中的作用入手，首先，明确HgS2基因为位于盐生草叶肉细胞液泡膜上的耐盐关键基因；接着，研究该基因调控叶肉细胞Na+液泡区隔化过程中的离子交换作用；最后，提取HgS2基因敲除株系和野生型盐生草植株叶肉细胞液泡膜蛋白，进行蛋白质组学分析，明确HgS2基因调控细胞Na+转运过程中的分子机制及其调控网络。本项目的实施能够为作物耐盐新品种培育提供基因资源和技术支撑。

Soil salinization has become one of the main threats restricting productivity to world agricultural production. Given the limited range of genetic diversity in salt tolerance within traditional crops, stress tolerance genes must be identified in extreme halophytes and then introduced into traditional crops. Halogeton glomeratus (H. glomeratus), is a succulent halophyte resources and native to arid regions in Northwest China. In this project, a new salt-tolerant gene HgS2 cloned from halophyte H.glomeratus be used as the material to analysis its functions in the transport process of Na+ compartmentalization into the vacuoles of mesophyll cells in H.glomeratus. Firstly, the HgS2 is identified as the key gene for salt tolerance on the vacuole membrane of mesophyll cells of H.glomeratus. Then, the characteristics of ion exchange in the process of Na+ vacuolar compartmentalization of mesophyll cells regulated by this gene are studied. Finally, we carry out the tonoplast proteome analysis of the HgS2 knock-out lines and wild type H.glomeratus leaves. The molecular mechanism and regulatory network of HgS2 in process of Na+ transport in plants will be constructed. The implementation of the project can provide gene resources and technical support for the cultivation of new salt tolerant crop varieties.