落粒性是影响作物产量的重要农艺性状，其精准调控有助于现代农业的生产管理和减少产量损失。虽然近年来克隆多个与水稻落粒性相关的重要QTL/基因，但是其复杂的调控网络仍不清楚。为进一步揭示水稻落粒性的遗传调控，本项目前期利用EMS诱变自然落粒的野生稻渗入系构建突变体库，并筛选到一个落粒性下降的突变体ssh1（suppression of shattering 1）。遗传分析表明该突变表型受一对隐性核基因控制。本项目拟在此基础上，采用图位克隆策略分离水稻落粒性调控基因SSH1，并验证其功能；进一步结合生物信息学和多种分子生物学分析方法，鉴定SSH1的互作基因和全基因组的结合位点，初步绘制其参与水稻落粒性遗传调控网络。研究结果不仅有助于揭示水稻落粒性调控的分子机制，而且将为水稻品种落粒性的分子选育提供重要参考。

Seed shattering is an important agronomic trait affecting crop yield, and precision regulation of seed shattering will be helpful for production management and reduction of yield loss in modern agriculture. Some important QTLs/genes associating with seed shattering have recently been identified in rice, however, their regulatory pathways still remain unknown. To identify new regulators of seed shattering, a wild rice introgression line with the seed shattering habit was mutagenized by ethyl methanesulfonate (EMS) to generate a library, in which a mutant with dramatic reduction of seed shattering, referred to as suppression of shattering 1 (ssh1), was identified. Further genetic analysis revealed that the non-shattering of ssh1 was controlled by a single recessive gene. On this basis, the aims of the proposed study are to isolate the SSH1 gene regulating seed shattering using map-based cloning approach, identify the interaction genes and genome-wide binding sites of SSH1 through an integrated strategy that combined bioinformatics and molecular biology analysis, and then construct the genetic network involving in the development of abscission zone and seed shattering. The results of the study will not only shed light on the genetic mechanism underlying seed shattering in rice, but also have great potential in crop improvement for avoiding yield loss.