有限的能源储备和日益加剧的环境压力，使得生物柴油的绿色高效转化获得越来越多的关注。酶促生物柴油转化具有化学法无法比拟的优势，然而目前发展的方法主要针对单一脂肪酶的催化性能调节。由于脂肪酶对甘油酯的区域选择性差异，导致脂肪酶的种类对不同原料油的生物柴油转化率有显著影响。为突破这一瓶颈，本项目拟将两种具有互补区域选择性的脂肪酶共同固定在同一载体上。为了降低不同酶分子间的抑制作用和固定化结构内部的传质阻力，结合磁性纳米粒子和金属-有机框架材料的优势，构建双层固定化酶模型，实现两种酶空间分离的共固定化。筛选得到活性高、稳定性好、易回收的固定化多脂肪酶复合酶。进而，用于废弃油脂的生物柴油转化，发展一种简单、绿色、高效的生物柴油合成方法。深入研究不同载体和固定化方法对脂肪酶酶活保留的影响规律。本项目的研究为多种脂肪酶的共同固定化提供新的思路，对推进生物柴油的绿色高效转化具有重要意义。

Due to limited energy reserves and increasing environmental pressures, green and efficient production of biodiesel has attracted more and more attention. In particular, enzymatic biodiesel production showed unique superiority over conventional chemical catalytic processes. Although many methods have been developed for enzymatic biodiesel production, they mainly aimed at the regulation of the catalytic performance of a single lipase. Because of the difference in regioselectivity and substrate specificity of lipases, lipase selection has a significant importance for biodiesel production from various renewable raw materials. To solve this problem, we try to construct hybrid magnetic metal-organic framework for efficient enzyme immobilization of two lipases showing different specificities, aiming at reducing the interference between different lipases and the internal mass transfer resistance in the microcapsules. On the basis of the above experiments, immobilized composite lipases with high activity, good stability and easy recovery will be obtained and further applied in biodiesel conversion of waste oils, developing a simple, efficient and green synthetic method for biodiesel production. Moreover, the coordination mechanisms of different functional groups on the activity of immobilized lipase will be studied in detail. This research is of great significance to promote green and efficient production of biodiesel and may provide a new idea for the establishment of efficient multi-enzyme immobilization.