“脾主肌肉”是筋伤骨病的核心理论之一，本团队长期应用推拿脾经治疗骨骼肌损伤，疗效显著。前期研究表明推拿脾经能降低腓肠肌钝挫伤大鼠TNF-a表达，促进肌肉修复。最新研究表明TNF-a可激活细胞内质网应激反应，诱导自噬，且内质网应激-自噬是骨骼肌损伤修复的关键，因此我们提出推拿脾经通过下调TNF-a表达调控内质网应激-自噬反应治疗骨骼肌损伤的假说。本项目拟以大鼠腓肠肌钝挫伤模型为研究对象，在“脾主肌肉”理论指导下，釆用推拿脾经治疗，运用WesternBlot、免疫电镜、免疫荧光、激光共聚焦扫描等技术，观察推拿脾经对TNF-a，骨骼肌内质网应激（钙离子、钙网蛋白酶、内质网应激蛋白），内质网自噬反应（自噬受体蛋白FMA134B、自噬标志性蛋白LC3）及应激蛋白与自噬蛋白相互作用（FMA134B与CRT、CRT与LC3表达量及共定位）的影响。探讨推拿脾经治疗骨骼肌损伤的作用机制，为临床应用奠定基础。

The theory of "spleen master muscle" is one of the core treatment theories of hamstring injury.And our team has applied massage of spleen meridian to treat sports injury for a long time,which has a significant clinical effect. Previous studies showed that massage of spleen meridian could reduce the expression of TNF-a in rats with blunt injury of gastriculus muscle and promote the repair of muscle injury. According to extensive research ,TNF-a can activate endoplasmic reticulum stress response and induce autophagy.And Endoplasmic reticulum stress - autophagy is the key to repair skeletal muscle injury.Therefore, A hypothesis is proposed that massage of spleen meridian can regulate endoplasmic reticulum stress-autophagy response by down-regulating TNF-a expression in the treatment of skeletal muscle injury.So in this project,we intends to use the model of blunt injury of gastrocnemius muscle in rats as research object and to treat with massage of spleen meridian under the guidance of the theory of “Splenic primary muscle”.Using ELISA, WesternBlot, Immunoelectron microscopy, Immunofluorescence and Laser confocal scanning to observe TNF-a,Calcium ion, calmodulin (SERCA, PDI), endoplasmic reticulum stress protein (CRT, GRP78) and CRT localization in autophagosome, localization and quantification of FMA134B and LC3.To explore the mechanism of massage of spleen meridian in the treatment of skeletal muscle injury and lay a foundation for clinical application.