脱氧雪腐镰刀菌烯醇（DON）的污染率及检出率较高，建立超灵敏、低成本的便携式定量检测方法在贫困地区、家庭保健防护、流通市场以及种植基地的DON检测中具有重大的发展潜力和应用价值。本项目拟采用Structure-Switching型核酸适体固定文库技术筛选高特异性、高亲和力的DON变构核酸适体，并借助计算机辅助3D分子模拟技术对起识别作用的重要功能基团进行修饰。然后，以变构核酸适体为特异性识别元件和交联剂，基于DNA碱基互补配对原则将α-葡萄糖苷酶封装于凝胶网格中构建智能“sweet”型DNA水凝胶体系，利用变构核酸适体-靶标相互作用，结合高效酶催化信号放大效应，配以高普及性的个人用血糖仪，建立一类核酸适体水凝胶-个人用血糖仪联用的新型便携式定量检测平台，从而实现靶标分子触发生成葡萄糖的信号转变机制，可为中药材和食品安全监控领域中各种有毒有害物质的检测提供重要的分析手段。

Deoxynivalenol (DON) is seriously contaminated and frequently detected, and thus is especially useful and attractive for developing more sensitive, lowcost, and portable detection method in the developing world, home healthcare settings, trading market and planting base monitoring. In this proposal, we intend to use Structure-Switching immobilized DNA library technology to screen allosteric aptamer with high specificity and high affinity, and then the important functional groups of the recognition function are further modified with the aid of computer-aided 3D molecular modeling technology. Afterwards, an intelligent “sweet” DNA hydrogel that uses allosteric aptamer as simultaneous crosslinking and specific recognition element was created on the base of base pairing, with alpha-glucosidase trapped inside the hydrogel network. Taking advantage of allosteric aptamer-target interactions, combined with catalytic signal amplification effect of highly efficient enzyme, a novel and portable quantitative detection platform is established by the combination of aptamer hydrogel and personal glucometer. Together with highly popular personal glucometer, the signal transformation mechanism of glucose triggered by the target molecule is achieved, which can be easily applied for detection of other chemical or biological targets in traditional Chinese medicine and food safety monitoring.