环境雌激素是环境中广泛存在的一大类内分泌干扰物，会对生态系统和人体健康造成很大威胁。目前通过传统的化学分离方法已经鉴定出了许多环境雌激素，但是在生产生活中仍有一些潜在的环境雌激素尚未被研究。比如说，再生水处理消毒过程中会产生许多未知的消毒副产物，已有研究表明部分消毒副产物具有雌激素干扰活性。使用传统化学分离方法来逐一研究这些消毒副产物的雌激素活性很困难。由于绝大多数环境雌激素都能和雌激素受体结合，因此本项目旨在通过建立一种基于雌激素受体蛋白的亲和层析方法，来特异性地分离再生水消毒副产物中可以与雌激素受体结合的组分，再利用化学方法进一步分离鉴定其中具有强雌激素干扰活性的新兴消毒副产物，并对它们的干扰活性和生成规律进行研究。这种新方法能够实现对再生水消毒后样品中强雌激素干扰活性物质进行一次性分析，并能发现未知的环境雌激素，为环境雌激素的有效控制提供科学依据和实验手段。

Environmental estrogens are the major endocrine disruptors which have done significant harm to the ecosystem and public health. The scientsits have already identified many estrogenic compounds by traditional chemical methods. However, there are still lots of unknown chemicals formed in the process of manufacture and daily life, which might have potential estrogenic activities. For example, thousands of disinfection by-products (DBPs) formed during the disinfection in the reclaimed water treatment process, and some of them had been identified as environmental estrogens. But it is difficult to study the estrogenicity of DBPs one by one by traditional way. Since most environmental estrogens can bind to the estrogen receptor, we attempt to establish an estrogen receptor based affinity chromatography to selectively isolate receptor binding compounds from disinfection products of reclaimed water. And then the DBPs with strong estrogenicity or anti-estrogenicity will be identified and characterized by the chemical methods. And we will also study their interference activities and formation at different disinfection conditions. This new method can analyze all high estrogenic disruptors in the disinfected reclaimed water together, screen out unknown estrogenic compounds, and support the strict regulation of environmental estrogens.