神经干细胞(NSCs)移植治疗脊髓损伤（SCI）具有美好的临床应用前景，而NSCs的定向分化在很大程度上决定其前景。有研究证实移植的NSCs选择性向神经元分化将更有利于SCI的修复，但其机制尚不明确。我们前期发现，沉默NF-1后可促进NSCs向神经元分化，并活化雷帕霉素靶蛋白复合物2(mTORC2)通路，而抑制mTORC2活性后，NF-1的沉默引起的NSCs向神经元分化的作用将减弱，综上提出科学假设:NF-1沉默的NSCs移植通过上调mTORC2信号通路促进细胞向神经元分化从而促进SCI修复。为验证该假设，本项目拟通过体内外实验探讨NF-1的沉默通过上调mTORC2通路促进NSCs向神经元分化的作用及机制，明确NF-1沉默的NSCs移植促进SCI修复的作用，为调控移植的NSCs向神经元定向分化提供新的基因修饰靶点。

Neural stem cells (NSCs) transplantation for the treatment of spinal cord injury (SCI) has a promosing prospect in the clinical application, however the directional differentiation of NSCs determines the available prospect in the clinical application. Research has demonstrated that the selective neuronal diffferntiation of transplanted NSCs is more beneficial to the repair of SCI, however, the mechanism remains unkown. Our previous work found that silencing NF-1 in NSCs could promote NSCs to differentiate into neurons, accompanied with activating the mTORC2 signaling pathway, moreover, the effect of promoting NSCs differentiate into neurons caused by NF-1 silencing would be attenuated after inhibition of the mTORC2 signaling pathway.Based on the above, we hypothesize that: NF-1 silencing NSCs transplantation promotes the NSCs to differentiate into neurons via up-regulating the mTORC2 activation, thereby facilitates the repair of SCI. In order to verify this hypothesis, we will investigate the effect and mechanism of that NF-1 silencing promotes NSCs to differentiate into neurons through up-regulating the mTORC2 activation. We try to definite the effect of NF-1 silencing NSCs transplantation on the repair of SCI. This study will provide a new gene modified target for the directional neuronal differentiation of transplanted NSCs.