尽管合成类糖皮质激素（synthetic glucocorticoids, sGCs）防治包括新生儿呼吸窘迫综合征、支气管肺发育不良等严重早产儿呼吸系统疾病的有效性已得到人们的认可，但对脑发育造成的潜在影响，使sGCs应用受到限制。现拟应用整体动物以及体外细胞培养制作地塞米松（dexamethasone，DEX）干预发育期大脑皮层神经元模型，研究sGCs对大脑皮层神经元迁移分子网络中LIS1信号通路、肾上腺皮质激素受体信号通路及神经元迁移运动的影响；并通过抑制糖皮质激素受体（glucocorticoid receptor，GR）在发育中皮层神经元的表达，研究GR与发育中大脑皮层神经元LIS1信号通路之间关系；进一步探究微小RNA（microRNA，miRNA）在DEX对发育中大脑皮层神经元迁移网络影响中的作用。以期阐明sGCs对发育中脑损害的机制，为更合理应用sGCs提供可能新的治疗。

Although synthetic glucocorticoids (sGCs) are effective to prevent neonatal respiratory distress syndrome and treat bronchopulmonary dysplasia in perinatal period, sGCs are limited as these agents can impair the brain development and cause the bad neurodevelopmental outcome. Therefore, we will produce developing rat cerebral cortex neurons injury with dexamethasone (DEX) models in vivo and in vitro. These models will be applied to investigate the effect of DEX on LIS1 signaling pathway, which is related to neurons migration, and adrenocorticoid receptor (ACR) signaling pathway in developing rat cerebral cortex. Next, we will inhibit glucocorticoid receptor (GR) expression with RNA interference or GR antagonist, and investigate the relationship between GR and LIS1 signaling pathway in developing rat cerebral cortex. Finally, we will explore whether microRNA plays an important role in the process which DEX intervents neural migration network. We expect to explain sGCs how to intervene brain development and find the better strategy to used sGCs in perinatal period.