脂肪分化相关蛋白（ADRP）是脂滴表面丰度较大的一个蛋白，而脂滴是丙肝病毒组装的关键细胞器。申请人在前期研究中发现GBF1 和ARF1 是病毒复制所需宿主因子，而ARF1 和ADRP 存在相互作用。本项目拟于丙型肝炎病毒感染细胞模型上，进一步研究ADRP 对丙肝病毒组装的调节机制：1）确定ADRP 限制丙肝病毒复制的准确步骤；2）阐明ADRP 与ARF1 相互作用对病毒复制的影响；阐明GBF1 与NS4B 相互作用对ARF1 活性及ADRP 定位的影响。3）阐明NS2 与ADRP 相互作用对于丙肝病毒组装的影响。本研究的展开将揭示ADRP 在丙肝病毒组装过程中的作用机制，具有重要的科学及临床意义。

Adipose differentiation related protein (ADRP) is an abundant protein decorated in the surface of lipid droplets (LD), which is a crucial organelle for Hepatitis C virus (HCV) assembly. Previously we found that GBF1 and ARF1 are host factors required for HCV replication and ARF1 interacts with ADRP. We propose three Specific Aims to pursue the mechanism by which ADRP regulates HCV assembly in HCV infectious model. Aim 1.Examine the precise step where ADRP restricts HCV replication. Aim 2. Dissect the impact of the interaction between ARF1 and ADRP on HCV replication. Dissect the impact of the interaction between GBF1 and NS4B on the activity of ARF1 and the location of ADRP. Aim 3. Dissect the impact of the interaction between NS2 and ADRP on the HCV assembly. This project will discover the mechanism by which ADRP regulate HCV assembly, which has scientific and clinical significance.